Protein Info for Sama_0031 in Shewanella amazonensis SB2B

Name: fadA
Updated annotation (from data): Acetyl-CoA C-acyltransferase (EC 2.3.1.16)
Rationale: Specifically important for utilizing Tween 20. Automated validation from mutant phenotype: the predicted function (KETOACYLCOATHIOL-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: 3-ketoacyl-CoA thiolase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 387 TIGR02445: acetyl-CoA C-acyltransferase FadA" amino acids 4 to 387 (384 residues), 730.2 bits, see alignment E=5.3e-224 PF00108: Thiolase_N" amino acids 5 to 254 (250 residues), 268.4 bits, see alignment E=6.4e-84 TIGR01930: acetyl-CoA C-acyltransferase" amino acids 6 to 385 (380 residues), 454.4 bits, see alignment E=3e-140 PF02803: Thiolase_C" amino acids 262 to 386 (125 residues), 163.4 bits, see alignment E=1.8e-52

Best Hits

Swiss-Prot: 100% identical to FADA_SHEAM: 3-ketoacyl-CoA thiolase (fadA) from Shewanella amazonensis (strain ATCC BAA-1098 / SB2B)

KEGG orthology group: K00632, acetyl-CoA acyltransferase [EC: 2.3.1.16] (inferred from 100% identity to saz:Sama_0031)

MetaCyc: 74% identical to 3-ketoacyl-CoA thiolase (Escherichia coli BL21(DE3))
Acetyl-CoA C-acyltransferase. [EC: 2.3.1.16]; 2.3.1.16 [EC: 2.3.1.16]; 2.3.1.16 [EC: 2.3.1.16]; 2.3.1.16 [EC: 2.3.1.16]

Predicted SEED Role

"3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9)" (EC 2.3.1.16, EC 2.3.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.16, 2.3.1.9

Use Curated BLAST to search for 2.3.1.16 or 2.3.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S1I7 at UniProt or InterPro

Protein Sequence (387 amino acids)

>Sama_0031 Acetyl-CoA C-acyltransferase (EC 2.3.1.16) (Shewanella amazonensis SB2B)
MKQAVIVDCIRTPMGRSKGGVFRNVRAETLSAELMKALLLRNPGVDPNTIEDVIWGCVQQ
TLEQGFNIARNASLLAGVPKTAGAVTVNRLCGSSMEALHQASRAIMTGMGDTFIIGGVEH
MGHVPMNHGVDFHPGLAANVAKASGMMGLTAEMLGKLHGISREMQDQFAVRSHQRAHAAS
IEGRFANEIYAIEGHDANGALIKVDYDEVIRPETTLESLAGLRPVFDPANGTVTAGTSSA
LSDGAAAMLVMEEEKARALGLTIRARVRSMAVAGCDAAIMGYGPVPATQKALARAGLSIQ
DMDVIELNEAFAAQSLPCVKDLGLMDVVEDKVNLNGGAIALGHPLGCSGARISTTLINLM
EHKDATLGLATMCIGLGQGIATVFERV