Protein Info for Shew_3756 in Shewanella loihica PV-4

Annotation: histidine ammonia-lyase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 94% identical to HUTH_SHEPA: Histidine ammonia-lyase (hutH) from Shewanella pealeana (strain ATCC 700345 / ANG-SQ1)

KEGG orthology group: K01745, histidine ammonia-lyase [EC: 4.3.1.3] (inferred from 100% identity to slo:Shew_3756)

MetaCyc: 70% identical to histidase subunit (Pseudomonas putida)
Histidine ammonia-lyase. [EC: 4.3.1.3]

Predicted SEED Role

"Histidine ammonia-lyase (EC 4.3.1.3)" in subsystem Histidine Degradation (EC 4.3.1.3)

MetaCyc Pathways

L-histidine degradation VI (7/8 steps found)
L-histidine degradation I (4/4 steps found)
L-histidine degradation III (4/6 steps found)
L-histidine degradation II (3/5 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.3

Use Curated BLAST to search for 4.3.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QJH4 at UniProt or InterPro

Protein Sequence (510 amino acids)

>Shew_3756 histidine ammonia-lyase (RefSeq) (Shewanella loihica PV-4)
MSHLVLKPGTLSLAQLRAISRGKVSLELCDSAVADINTSAGLVQKVLDEGRTVYGINTGF
GLLANTKIGEQDLQLLQRSIVLSHAAGTGQYMQDATVRLMMVLKINSLSRGFSGIRLEVI
NFLIALVNAEVYPCVPEKGSVGASGDLAPLSHMCLPLLGEGEMSYKGQIISAAEGLEIAG
LKPIELAAKEGLALLNGTQASTALALEGLFNAEDLFAASSVIGAMSVEAAMGSRSPFDPR
IHAARGQKGQIDSAAVFRHLLGDESEISLDHINCEKVQDPYSLRCQPQVLGACLTQIRHA
AEVLATEANGVTDNPLVFQDTGDIISGGNFHAEPVAMAADNLAIALAELGSIAERRIALL
IDSNLSKLPPFLVENGGVNSGFMIAQVTAAALASENKTYAHPASVDSLPTSANQEDHVSM
ATFAARRLRDMSENTRGVLAVELLAAAQGLDFRAPLQPATAVAKAKAELREVVAYYDKDR
FFGPDIEAATDLLLSASYNQYLPAGVLPSL