Protein Info for Shew_3530 in Shewanella loihica PV-4
Annotation: glycerol-3-phosphate acyltransferase (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to PLSB_SHELP: Glycerol-3-phosphate acyltransferase (plsB) from Shewanella loihica (strain ATCC BAA-1088 / PV-4)
KEGG orthology group: K00631, glycerol-3-phosphate O-acyltransferase [EC: 2.3.1.15] (inferred from 100% identity to slo:Shew_3530)MetaCyc: 52% identical to glycerol-3-phosphate 1-O-acyltransferase (Escherichia coli K-12 substr. MG1655)
Glycerol-3-phosphate O-acyltransferase. [EC: 2.3.1.15]; 2.3.1.15 [EC: 2.3.1.15]; 2.3.1.15 [EC: 2.3.1.15]; 2.3.1.15 [EC: 2.3.1.15]; 2.3.1.15 [EC: 2.3.1.15]
Predicted SEED Role
"Glycerol-3-phosphate acyltransferase (EC 2.3.1.15)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria (EC 2.3.1.15)
MetaCyc Pathways
- phosphatidylglycerol biosynthesis I (6/6 steps found)
- phosphatidylglycerol biosynthesis II (6/6 steps found)
- superpathway of phospholipid biosynthesis III (E. coli) (10/12 steps found)
- CDP-diacylglycerol biosynthesis I (4/4 steps found)
- CDP-diacylglycerol biosynthesis II (4/4 steps found)
- oleate biosynthesis III (cyanobacteria) (2/3 steps found)
- superpathway of cardiolipin biosynthesis (bacteria) (9/13 steps found)
- phosphatidate biosynthesis (yeast) (3/5 steps found)
- diacylglycerol and triacylglycerol biosynthesis (3/7 steps found)
- palmitoyl ethanolamide biosynthesis (2/6 steps found)
- superpathway of stearidonate biosynthesis (cyanobacteria) (2/6 steps found)
- stigma estolide biosynthesis (2/7 steps found)
- anandamide biosynthesis II (2/8 steps found)
- anandamide biosynthesis I (4/12 steps found)
- superpathway of phospholipid biosynthesis II (plants) (10/28 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.3.1.15
Use Curated BLAST to search for 2.3.1.15
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A3QIU8 at UniProt or InterPro
Protein Sequence (807 amino acids)
>Shew_3530 glycerol-3-phosphate acyltransferase (RefSeq) (Shewanella loihica PV-4) MSKQDSLWFKSLRWIQKKLVHTIVVPQDPFADLNLDSDRPLVYVMKTESISDIAALSEVT DRLGLPSPYQPLDVEGEQAPRVVCVEGAKPLLGEREGNEFYLESFSRLLSAHKKHPELDI QLVPVSLYWGRTPGKEDDSMKAAVLERESPTWLRKCLMIIFLGRHNFVQFSNAMSLRYMA DEHGTDKRIAQKLARVARVHFSRQRKVMTGPVLPNRQNLFHALLKSESMKKAILEEAQSK KISEAKARETAMEYLDEIAADYSDSLVRIAERFLTWLWNKLYKGINIKGAEQIRQLHHDG HEIVYVPCHRSHMDYLLLSYILYYQGMVPPHIAAGINLNFWPAGPMFRRGGAFFIRRSFR GNKLYTAVFREYLDQLFAKGYSVEYFTEGGRSRTGRLLAPKTGMIAMTLNSVLRGVERPV TLVPVYLGYDHVMEVATYHKELSGKKKQKESVWQVFGAIRKLGNFGQGYVNFGEPITLHS YLNEQVPEWREELAQDPEQKPSWLTPVVNTLANRVMTRINDAAAVSSITLTSLVLLASEQ NALQRSQLEKQLDLYLSLLKSVPYTGYTSVVEGDGAKLIAQGLELKKLKLDSDPLGDIIS IDESLAIAMTYYRNNIIHLLVIPSLIASCLTRQDECSRDEIIATVKDFYPLLQAELFMGI DNLETYVNQLLDQLLAEGLIDEDKHFSVKPDGLNQLLLLAGTISETMQRYAIIFNLLAAS PKIERSELESDSHQLAQRLGALHGITAPEFYDKKLYGTLSVKLKELGYLADDSHSEDVQR IRTRANSLLRSSVRQTIVDSVAAEHQA