Protein Info for Shew_3435 in Shewanella loihica PV-4

Annotation: nicotinate-nucleotide pyrophosphorylase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00078: nicotinate-nucleotide diphosphorylase (carboxylating)
  • amino acids 28 to 290 (263 residues), 322.2 bits, E=1.2e-100
• PF02749: QRPTase_N
  • amino acids 34 to 119 (86 residues), 90.5 bits, E=5.5e-30
• PF01729: QRPTase_C
  • amino acids 121 to 290 (170 residues), 212.3 bits, E=4e-67

Best Hits

Swiss-Prot: 61% identical to NADC_SALTY: Nicotinate-nucleotide pyrophosphorylase [carboxylating] (nadC) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K00767, nicotinate-nucleotide pyrophosphorylase (carboxylating) [EC: 2.4.2.19] (inferred from 100% identity to slo:Shew_3435)

MetaCyc: 60% identical to quinolinate phosphoribosyltransferase (decarboxylating) (Escherichia coli K-12 substr. MG1655)
Nicotinate-nucleotide diphosphorylase (carboxylating). [EC: 2.4.2.19]

Predicted SEED Role

"Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19)" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 2.4.2.19)

MetaCyc Pathways

• aspartate superpathway (24/25 steps found)
• NAD de novo biosynthesis I (5/6 steps found)
• NAD de novo biosynthesis IV (anaerobic) (5/6 steps found)
• NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde (3/4 steps found)
• NAD de novo biosynthesis III (4/6 steps found)
• NAD de novo biosynthesis II (from tryptophan) (4/9 steps found)
• nicotine biosynthesis (3/9 steps found)
• superpathway of nicotine biosynthesis (4/12 steps found)
• superpathway of NAD biosynthesis in eukaryotes (4/14 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Nicotinate and nicotinamide metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.19

Sequence Analysis Tools

See A3QIK3 at UniProt or InterPro

Protein Sequence (293 amino acids)

>Shew_3435 nicotinate-nucleotide pyrophosphorylase (RefSeq) (Shewanella loihica PV-4)
MLENDIRLAVKVALEEDLGFQADSNLTAEEKLSLDITAQLIPSDKYAQASLITREEGVFC
GKAWAEQVFNQLGGEVALHWHVDDGDLVLPNQVLCEMSGSARAILTGERTAMNFIQTLSG
VATLTKLYVDRLAGTHCKLLDTRKTIPGLRTAQKYAVTCGGGKNHRIGLFDAFLIKENHI
MASGSIASAINAARQLASDKLVEVEVESIDELKQALDAGADIVMLDNFDVTMMIEAVELN
NSYNENQRVKLEVSGNVTLDTISDYAKTGIDYISVGALTKHVKALDLSLRLKD