Protein Info for Shew_3412 in Shewanella loihica PV-4

Name: purH
Annotation: bifunctionalphosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00355: phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase
  • amino acids 8 to 529 (522 residues), 841 bits, E=1.9e-257
• PF02142: MGS
  • amino acids 19 to 132 (114 residues), 86.6 bits, E=1e-28
• PF01808: AICARFT_IMPCHas
  • amino acids 138 to 459 (322 residues), 425.1 bits, E=1.9e-131

Best Hits

Swiss-Prot: 100% identical to PUR9_SHELP: Bifunctional purine biosynthesis protein PurH (purH) from Shewanella loihica (strain ATCC BAA-1088 / PV-4)

KEGG orthology group: K00602, phosphoribosylaminoimidazolecarboxamide formyltransferase / IMP cyclohydrolase [EC: 2.1.2.3 3.5.4.10] (inferred from 100% identity to slo:Shew_3412)

MetaCyc: 76% identical to bifunctional AICAR transformylase/IMP cyclohydrolase (Escherichia coli K-12 substr. MG1655)
IMP cyclohydrolase. [EC: 3.5.4.10]; Phosphoribosylaminoimidazolecarboxamide formyltransferase. [EC: 3.5.4.10, 2.1.2.3]

Predicted SEED Role

"IMP cyclohydrolase (EC 3.5.4.10) / Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3)" in subsystem De Novo Purine Biosynthesis (EC 2.1.2.3, EC 3.5.4.10)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (45/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (25/26 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• inosine-5'-phosphate biosynthesis I (6/6 steps found)
• inosine-5'-phosphate biosynthesis II (5/5 steps found)
• inosine-5'-phosphate biosynthesis III (5/6 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• One carbon pool by folate
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.2.3 or 3.5.4.10

Sequence Analysis Tools

See A3QII0 at UniProt or InterPro

Protein Sequence (529 amino acids)

>Shew_3412 bifunctionalphosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (RefSeq) (Shewanella loihica PV-4)
MNNARPIRRALLSVSDKTGILEFAQALHAQGVELLSTGGTARLLADNGVPVIEVSDYTGH
PEIMDGRVKTLHPKVHGGILGRRGIDEIVMEQNAIKPIDLVAVNLYPFAETVAKEGCTLA
DAVENIDIGGPTMVRSTAKNHKDTTIVVNAKDYDRVIQEMQANQGSTTLETRFDLAIAAF
EHTAAYDGMIANYFGTMVPAHSQDECHQDSKFPRTYNTQLVKKQDLRYGENSHQSAAFYV
DLNIDEASVASATQLQGKALSYNNIADTDAALECVKEFSEPACVIVKHANPCGVAIGKDL
LEAYNRAYQTDPTSAFGGIIAFNGELDAETASAIVERQFVEVIIAPVVSQAARDVVAAKA
NVRLLECGQWASKTRSLDYKRVNGGLLIQDRDQGMVEMSDIKVVTKRQPTEAEMKDLMFC
WKVAKFVKSNAIVYAKDSMTIGVGAGQMSRVYSAKVAGIKAADENLEVVGSVMASDAFFP
FRDGIDAAAAAGISCIIQPGGSIRDEEIIAAADEHGMAMVFTGMRHFRH