Protein Info for Shew_3365 in Shewanella loihica PV-4

Annotation: acetyl-CoA carboxylase, biotin carboxylase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00289: Biotin_carb_N
  • amino acids 4 to 112 (109 residues), 150.5 bits, E=7e-48
• TIGR00514: acetyl-CoA carboxylase, biotin carboxylase subunit
  • amino acids 4 to 444 (441 residues), 687.5 bits, E=4.2e-211
• PF02655: ATP-grasp_3
  • amino acids 116 to 296 (181 residues), 34.2 bits, E=8.4e-12
• PF02786: CPSase_L_D2
  • amino acids 117 to 324 (208 residues), 260.9 bits, E=2.5e-81
• PF07478: Dala_Dala_lig_C
  • amino acids 143 to 293 (151 residues), 44.6 bits, E=3.7e-15
• PF02222: ATP-grasp
  • amino acids 145 to 294 (150 residues), 35 bits, E=3.3e-12
• PF02785: Biotin_carb_C
  • amino acids 338 to 444 (107 residues), 128.3 bits, E=4e-41

Best Hits

Swiss-Prot: 64% identical to ACCC_ECOLI: Biotin carboxylase (accC) from Escherichia coli (strain K12)

KEGG orthology group: K01961, acetyl-CoA carboxylase, biotin carboxylase subunit [EC: 6.3.4.14 6.4.1.2] (inferred from 100% identity to slo:Shew_3365)

MetaCyc: 64% identical to biotin carboxylase (Escherichia coli K-12 substr. MG1655)
Biotin carboxylase. [EC: 6.3.4.14]

Predicted SEED Role

"Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14)" in subsystem Fatty Acid Biosynthesis FASII (EC 6.3.4.14)

MetaCyc Pathways

• biotin-carboxyl carrier protein assembly (3/4 steps found)
• fatty acid biosynthesis initiation (type I) (1/3 steps found)
• jadomycin biosynthesis (3/9 steps found)
• 3-hydroxypropanoate cycle (5/13 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (8/18 steps found)
• glyoxylate assimilation (4/13 steps found)
• superpathway of the 3-hydroxypropanoate cycle (5/18 steps found)
• superpathway of mycolate biosynthesis (21/239 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis
• Propanoate metabolism
• Pyruvate metabolism
• Tetracycline biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.14, 6.4.1.2

Use Curated BLAST to search for 6.3.4.14 or 6.4.1.2

Sequence Analysis Tools

See A3QID3 at UniProt or InterPro

Protein Sequence (450 amino acids)

>Shew_3365 acetyl-CoA carboxylase, biotin carboxylase (RefSeq) (Shewanella loihica PV-4)
MRRFDKVLVANRGEIALRIIRACRQLGIKTVALYSSADKDSGHLRLADQQICIGPAPAKE
SYLNIAAILGAADLARVDAIHPGYGFLAENADFAQQVTDSGFHFIGPKADTIRLMGDKVS
AIAAMKAAGVPTVPGSDGLLGDNEAQSLEIAAQIGYPLIIKATAGGGGKGMRVVEDEAQL
LGAIALTRQEAEAAFGNGGVYLEKYLTRPRHIEVQVLCDHQGHCISLGERDCSLQRAQQK
VIESAPAPGLTPEQRQQISESCIRACQAIGYRGAGTLEFLYQDGEFYFMEMNTRIQVEHT
ISEMVTGVDLLVLQLRVAQGEPLALQESLIQPKGHAIECRINAEDPASFAPSPGRIDRLV
VPGGLGVRWESHLSPGAHVPPQYDAMIAKLIAWGDDRAQAIARMRTALDELTITGIKTNV
PLLRALLADPQVQQGETSIHYLEETFLPRR