Protein Info for Shew_3362 in Shewanella loihica PV-4

Annotation: hypothetical protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 51% identical to ARFB_ECOLI: Peptidyl-tRNA hydrolase ArfB (arfB) from Escherichia coli (strain K12)

KEGG orthology group: K15034, ribosome-associated protein (inferred from 100% identity to slo:Shew_3362)

MetaCyc: 51% identical to peptidyl-tRNA hydrolase, ribosome rescue factor (Escherichia coli K-12 substr. MG1655)
Aminoacyl-tRNA hydrolase. [EC: 3.1.1.29]

Predicted SEED Role

"Hypothetical protein YaeJ with similarity to translation release factor"

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.29

Use Curated BLAST to search for 3.1.1.29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QID0 at UniProt or InterPro

Protein Sequence (136 amino acids)

>Shew_3362 hypothetical protein (RefSeq) (Shewanella loihica PV-4)
MIKISQRVQIADNELEWQFIRSSGAGGQHLNKVSTAAQLIFDIRQSSLPEFYQQRLLAKT
DHRITKSGKIIIKCQASRSQDANRQTALEQFIALVASVGHTAKKRVATKPTKASQRRRVD
TKKQRGATKALRQKQF