Protein Info for Shew_3356 in Shewanella loihica PV-4

Annotation: CzcA family heavy metal efflux protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1076 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details transmembrane" amino acids 355 to 372 (18 residues), see Phobius details amino acids 379 to 398 (20 residues), see Phobius details amino acids 405 to 429 (25 residues), see Phobius details amino acids 480 to 500 (21 residues), see Phobius details amino acids 513 to 539 (27 residues), see Phobius details amino acids 571 to 590 (20 residues), see Phobius details amino acids 905 to 924 (20 residues), see Phobius details amino acids 931 to 951 (21 residues), see Phobius details amino acids 960 to 980 (21 residues), see Phobius details amino acids 1003 to 1022 (20 residues), see Phobius details amino acids 1034 to 1057 (24 residues), see Phobius details TIGR00914: heavy metal efflux pump, CzcA family" amino acids 1 to 1066 (1066 residues), 959.4 bits, see alignment E=1.1e-292 PF00873: ACR_tran" amino acids 7 to 1059 (1053 residues), 830.7 bits, see alignment E=1.6e-253 PF03176: MMPL" amino acids 849 to 1057 (209 residues), 21.8 bits, see alignment E=8.6e-09

Best Hits

KEGG orthology group: K07239, heavy-metal exporter, HME family (inferred from 100% identity to slo:Shew_3356)

Predicted SEED Role

"Cobalt-zinc-cadmium resistance protein CzcA; Cation efflux system protein CusA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QIC4 at UniProt or InterPro

Protein Sequence (1076 amino acids)

>Shew_3356 CzcA family heavy metal efflux protein (RefSeq) (Shewanella loihica PV-4)
MLQKLIDSAIRNRLLVALALLCGVVASVAMLPKLNLDAFPDVTNVQVTVNTAAEGLAAEE
VEKLISYPVESAMYALPSVTEVRSLSRTGLSIVTVVFAEGTDIYFARQQVFEQLQAAREM
IPDGVGVPEIGPNTSGLGQIYQYILRATPESGIDAAELRSLNDYLVKLIMMPVGGVTDVL
SFGGEVRQYQVQIEPNKLRSYGLSMAQVTQALESNNRNAGGWFMDQGQEQLVVRGYGLLP
AGEAGLAAIAQIPLTEAGGTPVRVADIAKVDFGAEIRVGAVTMTRRDDAGTPKALGEVVA
GVVLKRMGANTKATIDDISARTAMIEQALPKGVSFEVFYDQADLVNKAVETVRDALLMAF
LFIVVILALFLVNLRATLLVLLSIPVSIGLALMVMAYFGMSANLMSLGGLAVAIGMLVDG
SVVMVENIFKHLTQPDRRHFDQAKARASGESDPYHGEEDGLGEGERSGIALRVMLAAKEV
CSPIFFATAIIIVVFAPLFALEGVEGKLFQPMAVSIILAMVAALFVALIGVPALAVYLFK
RGVTLRESPVLKPVEALYRHLLSATMGRPKLVLLTAVTLFGISMALLPRLGTEFVPELEE
GTINLRVTLAPTASLSTSLDVAPKLEAMLMTFPEVDYALSRIGAPELGGDPEPVSNIEIY
IGLKPIEEWEHAETRLELQRKMEEKLSVFPGLLFTFSQPIATRVDELLSGVKAQLAIKLF
GPDLEVLSSKGQELSELVSGIEGAVDVSLEQVSGEAQLVVRPKRDLLARYGISVDEVMSL
VSQGIGGVSAGQVIDGNARYDINVRLASQYRSAPDALEDLLLSGVNGATVRLGEVADVVV
EMAPPNIRRDDVQRRVVVQANVSGRDMGSVVKDIYAIVPQADLPPGYTVVVGGQYENQQR
AQQKLMLVVPISIALIALLLYFSFGSIKQVGLIMANVPLALIGGVVALYVSGTYLSVPSS
IGFITLFGVAVLNGVVLVDSINQRRASGEALYTSVYEGTVGRLRPVLMTALTSALGLIPI
LLSSGVGSEIQKPLAVVIIGGLFSSTALTLLVLPTLYRWLYETKALKGDKELEALD