Protein Info for Shew_3335 in Shewanella loihica PV-4

Annotation: nitrite reductase, copper-containing (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 392 signal peptide" amino acids 18 to 19 (2 residues), see Phobius details transmembrane" amino acids 20 to 41 (22 residues), see Phobius details TIGR02376: nitrite reductase, copper-containing" amino acids 62 to 386 (325 residues), 493.7 bits, see alignment E=1.4e-152 PF07732: Cu-oxidase_3" amino acids 94 to 208 (115 residues), 50.6 bits, see alignment E=4.1e-17 PF07731: Cu-oxidase_2" amino acids 119 to 207 (89 residues), 23.2 bits, see alignment E=1e-08 PF00394: Cu-oxidase" amino acids 224 to 375 (152 residues), 55.2 bits, see alignment E=1.9e-18

Best Hits

Swiss-Prot: 59% identical to NIRK_RHIME: Copper-containing nitrite reductase (nirK) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00368, nitrite reductase (NO-forming) [EC: 1.7.2.1] (inferred from 100% identity to slo:Shew_3335)

Predicted SEED Role

"Copper-containing nitrite reductase (EC 1.7.2.1)" in subsystem Denitrification (EC 1.7.2.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.7.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QIA3 at UniProt or InterPro

Protein Sequence (392 amino acids)

>Shew_3335 nitrite reductase, copper-containing (RefSeq) (Shewanella loihica PV-4)
MEKQSSTPAVNVDQQRRSFLSGSVGLGLVGAGMAAGLVSSSASATTQTKSDRMANYKAEQ
LARVTLDLVPPPMVPKHDQVAKGKPKVVQVKLVVEEKQVELENGTKAWVCAFNGSVPGPL
IVCHQHDFVELTLVNPATNALAHNIDLHAATGALGGGELTLVSPGQEVTFRFKATKAGTF
VYHCAPGGVMIPWHVAMGMHGAIMVLPRDGLKDAEGKPVKYDKAYYIGEADFYVPKDANG
KYKAYDTVMGSMSEVLEVMKTLTPSHVVFNGAVGALTGKNAMTAKVGESVLFVHSQANRD
TRPHIIGGHGDYVWPAGSFTDAPQTNLETWMVAGGSACAALYTFRQPGLYAYVNHNLIEA
IIKGAAAHVKVDGEWDDNLMVQLKKPSSIATA