Protein Info for Shew_3003 in Shewanella loihica PV-4

Name: fadE
Annotation: acyl-CoA dehydrogenase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 744 PF02771: Acyl-CoA_dh_N" amino acids 70 to 163 (94 residues), 50.6 bits, see alignment E=4.7e-17 PF02770: Acyl-CoA_dh_M" amino acids 167 to 267 (101 residues), 38.3 bits, see alignment E=2.5e-13 PF00441: Acyl-CoA_dh_1" amino acids 290 to 430 (141 residues), 47.1 bits, see alignment E=5.7e-16 PF09317: ACDH_C" amino acids 444 to 728 (285 residues), 233 bits, see alignment E=1.1e-72

Best Hits

KEGG orthology group: K00249, acyl-CoA dehydrogenase [EC: 1.3.99.3] (inferred from 100% identity to slo:Shew_3003)

Predicted SEED Role

"Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2)" in subsystem Isoleucine degradation (EC 1.3.99.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.2, 1.3.99.3

Use Curated BLAST to search for 1.3.99.2 or 1.3.99.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QHC1 at UniProt or InterPro

Protein Sequence (744 amino acids)

>Shew_3003 acyl-CoA dehydrogenase (RefSeq) (Shewanella loihica PV-4)
MSIRTKFKKVLPSISTTEQEALDAGDVWLEGSIYQGIPDFDALRQVPGATLSDEEQAFLD
GPVQTLIEMVDDFAIQNSLHLPDNILNFLKEHKFFSLIIPKAFGGLEFSPYANSTIVATI
AAKSSAVAVTVMVPNSLGPGELLMHYGTQAQQDFWLPRLANGQEIPCFALTSPEAGSDAG
GIPDIGTVTMGEYQGEQVLGLSVTWDKRYITLAPIATVLGLAFKVEDPQGLLGGKEQLGI
TCALIPKSHPGVELGNRHDPMGCRFYNGTTRGENVFIPMDFIIGGQQNIGRGWQMLVACL
GAGRGISLPALGVSVSQASFKSSAEYAAVREQFGLAIGKFEGIQEKLADIAGKTYLQEAM
RVLTTEGLGLGLKPSVVTAMAKYHMTEIGRDVLNSAMDIQAGKAIQRGPQNTLASGYVAQ
PIAITVEGANILTRNLMIFGQGVMRCHPHLQSMVEAIHSDDKDADKTFNRIFKQTVGYSV
GNSLRAFRLGLLPFTADAKSELSEVVEYEKAVHKLASKLAVYADFSLLVLGGKLKQAEML
SARLGDVMSYLYAAMASIRYYEQKVSSEHRAQAKPYFEYATRWALVNAENALLSFLDNFP
SAATRQLMKLITVTYSPKMKKINDDLIRELAHEAQLNTQIKRQLTHLVKPVPGDGNDINQ
QAYLAKIACLPLLAKVKKGLKQGYFKSGVRFAQTLDRAREAKIITEGEHKQLLDYNLKRE
RAIRVDEFDFDMQLISDKAALKIA