Protein Info for Shew_2881 in Shewanella loihica PV-4

Annotation: Na(+)-translocating NADH-quinone reductase subunit A (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 444 PF05896: NQRA_N" amino acids 2 to 95 (94 residues), 131.2 bits, see alignment E=1.9e-42 TIGR01936: NADH:ubiquinone oxidoreductase, Na(+)-translocating, A subunit" amino acids 2 to 444 (443 residues), 590.7 bits, see alignment E=9.5e-182 PF24836: NQRA_2nd" amino acids 116 to 254 (139 residues), 200.5 bits, see alignment E=2.2e-63 PF11973: NQRA_SLBB" amino acids 259 to 308 (50 residues), 69.3 bits, see alignment 4.8e-23

Best Hits

Swiss-Prot: 85% identical to NQRA_SHEFN: Na(+)-translocating NADH-quinone reductase subunit A (nqrA) from Shewanella frigidimarina (strain NCIMB 400)

KEGG orthology group: K00346, Na+-transporting NADH:ubiquinone oxidoreductase subunit A [EC: 1.6.5.-] (inferred from 100% identity to slo:Shew_2881)

MetaCyc: 65% identical to Na(+)-translocating NADH-quinone reductase subunit A (Vibrio cholerae)
TRANS-RXN-214 [EC: 7.2.1.1]

Predicted SEED Role

"Na(+)-translocating NADH-quinone reductase subunit A (EC 1.6.5.-)" in subsystem Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes (EC 1.6.5.-)

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.-

Use Curated BLAST to search for 1.6.5.- or 7.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QGZ9 at UniProt or InterPro

Protein Sequence (444 amino acids)

>Shew_2881 Na(+)-translocating NADH-quinone reductase subunit A (RefSeq) (Shewanella loihica PV-4)
MITIKKGLDLPITGGPEQVIHNGPAIKHVATLGEEYIGLRPTMKIKVGDKVQKGQVIFED
KKNPGVKYTALASGTISEINRGAQRVLQSVVIEVEGDDSVSFAKYDAAALDTLEPQLVRD
NLIESGMWPALRTRPFSKAPAVDSTPAGIFVTAIDTQPLAADPAVIINEHKEDFANGLRV
LARLTEGKVYLCKAPGADIPAANAQVEEFAGPHPAGLAGTHIHFVLPASAKRTVWHVGYQ
DVIAIGQLFTTGELNTQRVVAIAGPKALKPRLVRTHSGASIAELTAGETAQGDVRVIAGS
VLSGKTATGPHAYLGRFFNQVSLLEEGREQEFIGWAMPGANKFSITRAFLGHLSPSRLFN
MTTSTGGSDRAMVPIGNYERVMPLDILPTMLLRDLVSGDTDGAAALGALELDEEDLALCT
FVCPGKYDYASYLRDCLDTIVREG