Protein Info for Shew_2720 in Shewanella loihica PV-4

Annotation: peptidase S8 and S53, subtilisin, kexin, sedolisin (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 613 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF00082: Peptidase_S8" amino acids 126 to 341 (216 residues), 97.9 bits, see alignment E=6.9e-32 amino acids 414 to 501 (88 residues), 55.1 bits, see alignment E=7.7e-19 PF02225: PA" amino acids 363 to 431 (69 residues), 44.6 bits, see alignment E=1.3e-15

Best Hits

KEGG orthology group: None (inferred from 100% identity to slo:Shew_2720)

Predicted SEED Role

"Cold-active alkaline serine protease (EC 3.4.21.62)" (EC 3.4.21.62)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.62

Use Curated BLAST to search for 3.4.21.62

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QGI8 at UniProt or InterPro

Protein Sequence (613 amino acids)

>Shew_2720 peptidase S8 and S53, subtilisin, kexin, sedolisin (RefSeq) (Shewanella loihica PV-4)
MKLTKLASAVMALSVGLSTQAFADDDRYIIQVDNGSKGVVKALAKQLGAQIHVDGEGFIA
ATFAGKDLAQVKGLLNNPHVKLVEADQRRHLMSFSDDAGNPMTQQVTPYAVYQSQANQLT
FNAAAGMKVCIIDSGLDASNPDFEWNKITGDNDSGTGSWNVNGGPHGTHVAGTIGAADNN
VGVIGMAPGVDMHIIKVFNAEGWGYSSDLAHATNLCSAAGANIISMSLGGGGANSTESNA
FQAFVDAGGLVVAAAGNDGNNVRSYPAGYPSVMMVGANDANNNIADFSQFPSCTSGRGPK
ATSDETICVEVTAGGVDTLSTYPAGMATASNLSADGASFASSAMENSGTVSGNTYYMGTA
EATDAGANGNICVIDRGVISFHDKVANCENSGGVGAIIINNEPGMLYGTLGDTNTTSIPA
VGTAQEDRAALLAASTATVDIGTADYGFMSGTSMATPAVSGIAALVWSNHNQCTGSEIRA
ALKATAMDSGAPGKDEYFGYGIVQAAAADAYLNANGCAGGDNGGGDNGGGNTGGDFTLSA
NGYKNKGVKTVDLTFSGAAGANVDVYRDGSKLATTSASSTYTDTINTKGGGTYVYKACDE
GTSTCTAEQTVIF