Protein Info for Shew_2675 in Shewanella loihica PV-4

Annotation: crotonobetainyl-CoA dehydrogenase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 378 PF02771: Acyl-CoA_dh_N" amino acids 6 to 108 (103 residues), 61 bits, see alignment E=2.7e-20 PF02770: Acyl-CoA_dh_M" amino acids 122 to 213 (92 residues), 71.8 bits, see alignment E=8.9e-24 PF00441: Acyl-CoA_dh_1" amino acids 225 to 374 (150 residues), 154 bits, see alignment E=6.6e-49 PF08028: Acyl-CoA_dh_2" amino acids 243 to 354 (112 residues), 49.5 bits, see alignment E=1.1e-16

Best Hits

Swiss-Prot: 81% identical to CAIA_ECO8A: Crotonobetainyl-CoA reductase (caiA) from Escherichia coli O8 (strain IAI1)

KEGG orthology group: K08297, crotonobetainyl-CoA dehydrogenase [EC: 1.3.99.-] (inferred from 100% identity to slo:Shew_2675)

MetaCyc: 80% identical to crotonobetainyl-CoA reductase (Escherichia coli K-12 substr. MG1655)
CROBETREDUCT-RXN [EC: 1.3.8.13]

Predicted SEED Role

"Crotonobetainyl-CoA dehydrogenase (EC 1.3.99.-)" (EC 1.3.99.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.-

Use Curated BLAST to search for 1.3.8.13 or 1.3.99.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QGE3 at UniProt or InterPro

Protein Sequence (378 amino acids)

>Shew_2675 crotonobetainyl-CoA dehydrogenase (RefSeq) (Shewanella loihica PV-4)
MDFKLTDEQELFVSGIRDLMAKENWESYFAECDENSQYPERFVKELAEMGIDSLLLPEEH
GGFEEGMVTLTAIWEELGRLGAPTYVLYQLPGIATVLRHGNQDQIDKIMALRGTGKQMWN
SAITEPSAGSDVGSLLTTYKRRNGKVYLTGSKCFITSAAGIPYVVVMAKDADSEKPIFTE
WFVDMTKPGIKLSKLPKLGLKMDSCCELQFDEVELEESDMFGIEGNGFLRVKEEFDSERF
LVALTNYGAAYCAFEDAAKYANQRVQFGETIGRFQLIQDKFAHMAIKLNSMKNMVYETAW
KCDQGTMTSGDSAMCKYFCANAGFEVIDSAMQVLGGYAIAGEHRISRMWRDIRVDRVSGG
SDEMQILTLGRAVLKQYR