Protein Info for Shew_2534 in Shewanella loihica PV-4
Annotation: glycerate kinase (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 58% identical to GLXK_BACSU: Glycerate kinase (glxK) from Bacillus subtilis (strain 168)
KEGG orthology group: K00865, glycerate kinase [EC: 2.7.1.31] (inferred from 100% identity to slo:Shew_2534)MetaCyc: 56% identical to glycerate 2-kinase 1 (Escherichia coli K-12 substr. MG1655)
GKI-RXN [EC: 2.7.1.165]
Predicted SEED Role
"Glycerate kinase (EC 2.7.1.31)" in subsystem Allantoin Utilization or D-galactarate, D-glucarate and D-glycerate catabolism or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or Glycine and Serine Utilization or Photorespiration (oxidative C2 cycle) or Serine-glyoxylate cycle (EC 2.7.1.31)
MetaCyc Pathways
- photorespiration II (8/10 steps found)
- photorespiration I (7/9 steps found)
- photorespiration III (7/9 steps found)
- glycolate and glyoxylate degradation I (3/4 steps found)
- superpathway of glycol metabolism and degradation (5/7 steps found)
- 2-O-α-mannosyl-D-glycerate degradation (1/2 steps found)
- D-glucarate degradation I (2/4 steps found)
- D-galactarate degradation I (2/4 steps found)
- formaldehyde assimilation I (serine pathway) (8/13 steps found)
- Entner-Doudoroff pathway II (non-phosphorylative) (5/9 steps found)
- superpathway of D-glucarate and D-galactarate degradation (2/5 steps found)
- 2-deoxy-D-ribose degradation II (3/8 steps found)
- superpathway of microbial D-galacturonate and D-glucuronate degradation (7/31 steps found)
KEGG Metabolic Maps
- Glycerolipid metabolism
- Glycine, serine and threonine metabolism
- Glyoxylate and dicarboxylate metabolism
Isozymes
No predicted isozymesUse Curated BLAST to search for 2.7.1.165 or 2.7.1.31
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A3QG02 at UniProt or InterPro
Protein Sequence (381 amino acids)
>Shew_2534 glycerate kinase (RefSeq) (Shewanella loihica PV-4) MKIVIAPDSYKESLSAMEVATEIARGFCEVLPDAEYIKLPVADGGEGTVQSMVDATGGKL VELKVTGPLGSQVDAHYGLLGQEAPGQRTAVIEMASASGLHHVPTLLRDPLVTTSYGTGE LICHALNSGVTHLILGLGGSATNDGGAGMLQALGAHLLDNEGKPLRPGGAALEHLASVDI GELHPRLAQVEIEVACDVDNPLCGDKGASAIFGPQKGADEIMVARLDNALSHFADITSSV GLKECRNMPGAGAAGGMGFAMLAYLGAKLRPGIDIVMETVRLSEHLKGADLVITGEGRLD SQTLHGKTPMGVTREANKQGIPVIAIAGCVSEDANVLLDHGIDALFSVTPRALPLEEVLA GARHNLYSCAVNIARLYRLGR