Protein Info for Shew_2254 in Shewanella loihica PV-4
Annotation: bifunctional indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 47% identical to TRPC_HELPJ: Tryptophan biosynthesis protein TrpCF (trpC) from Helicobacter pylori (strain J99 / ATCC 700824)
KEGG orthology group: K13498, indole-3-glycerol phosphate synthase / phosphoribosylanthranilate isomerase [EC: 4.1.1.48 5.3.1.24] (inferred from 100% identity to slo:Shew_2254)Predicted SEED Role
"Indole-3-glycerol phosphate synthase (EC 4.1.1.48) / Phosphoribosylanthranilate isomerase (EC 5.3.1.24)" in subsystem Tryptophan synthesis or Auxin biosynthesis (EC 4.1.1.48, EC 5.3.1.24)
MetaCyc Pathways
- superpathway of chorismate metabolism (51/59 steps found)
- superpathway of aromatic amino acid biosynthesis (18/18 steps found)
- superpathway of L-tryptophan biosynthesis (13/13 steps found)
- L-tryptophan biosynthesis (6/6 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Phenylalanine, tyrosine and tryptophan biosynthesis
Isozymes
No predicted isozymesUse Curated BLAST to search for 4.1.1.48 or 5.3.1.24
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A3QF72 at UniProt or InterPro
Protein Sequence (483 amino acids)
>Shew_2254 bifunctional indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase (RefSeq) (Shewanella loihica PV-4) MQASVNSQTGPSNTSSNVLTRIVDTKPAHIAALKQRFPEASLAPKVSDRSLYQALKAPNA GFIFECKKASPSKGLIRQAFDVEAIADVYNHYAAAISVLTDEQFFQGDMDYLPKVRARVS QPILCKDFFVDPYQVKLAAHQGADAILLMLSVLDDDHYRQLASLAAQYQLDVLTEVSNEE ELKRAIALDAAIIGINNRNLRDLTTDLATTEALAPHIPADRVVVSESGIYTQQQVRRLSP LVDGFLVGSSLMAEADLDLACRALTLGHNKVCGLTRSEDVRAVIDAGALYGGLIFAKKSP RYVSPEQALDLVEQQRSSGKGLNFVGVFVNEELSTIAELAKRLNLFAVQLHGSESEYDID QLRFKLNELDCQTQIWKAVAVSVDSDDCQAPSTPANADRLLYDSKTKATDGVQFGGTGHA FNWQQQLPNKADAMLAGGLNAGNAFDAARQGFFGLDFNSGLESAPGVKDAELIKQAFTAL REY