Protein Info for Shew_2204 in Shewanella loihica PV-4

Annotation: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01502: PRA-CH
  • amino acids 33 to 105 (73 residues), 108.6 bits, E=1.1e-35
• TIGR03188: phosphoribosyl-ATP diphosphatase
  • amino acids 115 to 197 (83 residues), 102.7 bits, E=4.9e-34
• PF01503: PRA-PH
  • amino acids 116 to 201 (86 residues), 67.7 bits, E=9.6e-23

Best Hits

Swiss-Prot: 80% identical to HIS2_SHEON: Histidine biosynthesis bifunctional protein HisIE (hisI) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K11755, phosphoribosyl-ATP pyrophosphohydrolase / phosphoribosyl-AMP cyclohydrolase [EC: 3.5.4.19 3.6.1.31] (inferred from 100% identity to slo:Shew_2204)

MetaCyc: 61% identical to putative bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase (Escherichia coli K-12 substr. MG1655)
Phosphoribosyl-ATP diphosphatase. [EC: 3.6.1.31]; Phosphoribosyl-AMP cyclohydrolase. [EC: 3.6.1.31, 3.5.4.19]

Predicted SEED Role

"Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) / Phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31)" in subsystem Histidine Biosynthesis (EC 3.5.4.19, EC 3.6.1.31)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (45/46 steps found)
• L-histidine biosynthesis (10/10 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• Histidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.4.19 or 3.6.1.31

Sequence Analysis Tools

See A3QF22 at UniProt or InterPro

Protein Sequence (206 amino acids)

>Shew_2204 bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein (RefSeq) (Shewanella loihica PV-4)
MKNLEDFNNLDWDKQQGLLPAVVQNHLTGKVLMLGYMNQEALAQTLASRKITFFSRSKQR
LWTKGETSGNTLDLIAIDSDCDNDSLLVQVIPNGPTCHLERESCWPDGEAHPFIDNLANL
IASRRGQDAKSSYTAHLFERGTKRIAQKVGEEGLETALAAATHDKEELINEASDLIYHLL
VLLEDQDLSLEDITANLLARHSAASK