Protein Info for Shew_2171 in Shewanella loihica PV-4

Annotation: peptidase T2, asparaginase 2 (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 348 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF01112: Asparaginase_2" amino acids 30 to 345 (316 residues), 398.6 bits, see alignment E=8.7e-124

Best Hits

Swiss-Prot: 51% identical to IAAA_SALTY: Isoaspartyl peptidase (iaaA) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K13051, beta-aspartyl-peptidase (threonine type) [EC: 3.4.19.5] (inferred from 100% identity to slo:Shew_2171)

Predicted SEED Role

"Isoaspartyl aminopeptidase (EC 3.4.19.5) @ Asp-X dipeptidase" (EC 3.4.19.5)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.19.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QEY9 at UniProt or InterPro

Protein Sequence (348 amino acids)

>Shew_2171 peptidase T2, asparaginase 2 (RefSeq) (Shewanella loihica PV-4)
MRSTITPSLLAGLMGLLISTSTVAETQPFSIAIHGGAGTISKANLTEAQQQAYRDKLKEA
VDAGYKVLEKGGDSLTAVTTAINILEDSPLFNAGKGAVYTYDGTHEMDASIMDGRNLNAG
AVAGVKHIKNPINLARAVMDKSPHVMLSGQGAEEFALSQDFSLVPVTYFDTESRYQQLID
AKAKLKAAESKEAGKPDYQASVNYLDLDYKFGTVGAVALDKQGNLAAGTSTGGMTVKRFG
RIGDSPVIGAGTYAENQVCAVSATGHGEYFIRYHVAGDICAKVKYQQKSILQAADEVINQ
RLITAGGTGGVIAIDQRGNIATPFNTEGMYRATRKGGEPAKVMIWQDN