Protein Info for Shew_2162 in Shewanella loihica PV-4

Name: cpdB
Annotation: bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic precursor protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 694 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details TIGR01390: 2',3'-cyclic-nucleotide 2'-phosphodiesterase" amino acids 37 to 688 (652 residues), 887.6 bits, see alignment E=2.5e-271 PF00149: Metallophos" amino acids 39 to 284 (246 residues), 43.8 bits, see alignment E=4.1e-15 PF02872: 5_nucleotid_C" amino acids 391 to 585 (195 residues), 48.6 bits, see alignment E=9.3e-17

Best Hits

KEGG orthology group: K01119, 2',3'-cyclic-nucleotide 2'-phosphodiesterase [EC: 3.1.4.16] K08693, 3'-nucleotidase [EC: 3.1.3.6] (inferred from 100% identity to slo:Shew_2162)

Predicted SEED Role

"2',3'-cyclic-nucleotide 2'-phosphodiesterase (EC 3.1.4.16)" in subsystem Purine conversions (EC 3.1.4.16)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.3.6 or 3.1.4.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QEY0 at UniProt or InterPro

Protein Sequence (694 amino acids)

>Shew_2162 bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic precursor protein (RefSeq) (Shewanella loihica PV-4)
MDMFNKKLLAVVIASSIGLTACSWNDDDEDTITSANVELRVLETTDLHTNIMDYDYYQSK
VDPTIGLARTASLIHAARKEATNFVLVDNGDLLQGSPMGDYMAKIGLKEGDVHPAYKAMN
TLDYEVANIGNHEFNYGLDFLEKALSGAEFPYINANVYCAADDCWNDIKKGDNLFTPYLI
KEKVVIDTQGREHTIKIGYIGFVPPQIMLWDKQHLTGKVEAKDIIETAKLFVPKMKAEGA
DVIIAIPHSGIGSTENPGDAMAENATFALTNVEGIDAIMFGHSHSIFPHANYADLPNTDV
EKGLLNGVAAVMPGRWGDNLGVVDLKLTLKDGAWSVTDAQTEARPIYDGANKVALVDADK
DIHDAVELEHQGTLAFVDQPIGVAAADMYSFLTLVQDDPTVQIVSDAQIAQVSAKLPESL
QGIPVLSAAAPFKAGGRHATEADASQFVQVDAGDLTFKNAADLYLYPNTMVAVKITGVEL
KDWLECSANQFNQINPNSSEPQSLINWNGHPTYNFDVIDGVTYKIDVTQPSKFDRDCAVI
AGNENAQRIVDLAYTTPTGEVLTGDALSAQEFIVASNNYRAFGGKFAGTGSEYVVMELPD
TNREALAAYITAQSKPNDQGGYDGMVDPRADYNWDFKTINTQVALDIRFETQNSDKADKF
ISDNQLRKMEKLAEPDALGFAIYRIDLTSDPVAK