Protein Info for Shew_2158 in Shewanella loihica PV-4

Annotation: gamma-glutamyltransferase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 576 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details TIGR00066: gamma-glutamyltransferase" amino acids 45 to 567 (523 residues), 645.5 bits, see alignment E=2.8e-198 PF01019: G_glu_transpept" amino acids 60 to 570 (511 residues), 605.8 bits, see alignment E=3.9e-186

Best Hits

KEGG orthology group: K00681, gamma-glutamyltranspeptidase [EC: 2.3.2.2] (inferred from 100% identity to slo:Shew_2158)

Predicted SEED Role

"Gamma-glutamyltranspeptidase (EC 2.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle or Utilization of glutathione as a sulphur source (EC 2.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QEX6 at UniProt or InterPro

Protein Sequence (576 amino acids)

>Shew_2158 gamma-glutamyltransferase (RefSeq) (Shewanella loihica PV-4)
MRPIKTLLFAISLSLPLVAGQFAQATESSIYSHSATVQPIWAKHGMVASQEAVATQVGVD
ILKQGGNAVDAAVGVGFALAVTLPRAGNIGGGGFMLVHLAEGNKTVAIDYREVAPKRAHK
DIFLNEQGDAVAKLSREHGLAVGVPGTVMGMELALKKYGTMTMAQVIEPAIKLAEQGITV
TPDLATSLAGLKRRISQWPSSKAIFYRPDGSNFEIGDTLSQKALAHSLKLIKAQGSKGFY
EGETAEKIVASVTQAGGIMTLDDLKGYRVVERQPVQGEYRGYQVISMPPPSSGGIHIIEM
LNMLEPYPIAKLGHNTADTLHLMAEVMKRAYADRSEYLGDPDFVEVPVKALISKDYAKSR
AKGIAINRVTPSSEIAPGKLAGYESPQTTHYSVVDKWGNAVANTYTLNFSYGSGLVAEGT
GILLNNEMDDFSAKPGVPNGYGLIGGDANAVEGGKRPLSSMSPTIVMKDGKPFIVTGSPG
GSRIINTTLQIIMNVIDHGLNIAEASYASRVHHQWLPDELRVEKSLNRDTISLLEARGHK
VSVKDAMGSTQSIMITDEGLFGASDPRRAGSATAGY