Protein Info for Shew_2065 in Shewanella loihica PV-4

Annotation: diguanylate cyclase/phosphodiesterase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 642 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details transmembrane" amino acids 135 to 154 (20 residues), see Phobius details PF00990: GGDEF" amino acids 220 to 370 (151 residues), 35.3 bits, see alignment E=9.7e-13 PF00563: EAL" amino acids 397 to 625 (229 residues), 173.5 bits, see alignment E=5e-55

Best Hits

KEGG orthology group: None (inferred from 100% identity to slo:Shew_2065)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains)"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QEN3 at UniProt or InterPro

Protein Sequence (642 amino acids)

>Shew_2065 diguanylate cyclase/phosphodiesterase (RefSeq) (Shewanella loihica PV-4)
MGVGKSIHLLLITACLALTALAYLNQRQEIAAIADFQLKEYQADPIWQQPLPEQGQALFT
LLTKQYEFQFFQYVHQLDGNLNYTQGELIASGTDLATVLFNQPRENTQALPQGRLQVKLS
SRTIAKDALTHFTQTLIWIVGGYLALAVLFTLLMARQKRTIGYAVNALDGFAELKFDAIA
HSRFHGELKPLGKALEHCRVALHDKFDQLQQQTEQLNREAYQDPVTLFGTRPKFTEKLDA
LAKPGKSRIGLMAMIKATELGHVNQSQGRAAGDDYLAGVATCIRKALQGMPNIEAFRISS
ADFAVFIPDLVLKDAQQPLEKLKAQFDEYQQGSDLESVAYIGMVPYEQGGDALSLVYIAD
AAVSIAQTLGPNSYHVQEKLSDEELVGDSHWKLAIEDIIKRRAIKFHAQDIQPCRGADNV
YRELLSRFYSSEGKFLPTGTVIAMAERHGLSVELDKIIILSTLRLLLDTPTFTGALGINI
STASVNQPAFTAWLKDIFVRQRQIASRLVLEVNEAGMQANIRQAHKFVRDMHSVGARVAV
EHFGMGFTSFKFFKEVRPDFIKLDGSYSQGIDTDSNNRFFVKMIVDVARKQSVKVIASSI
ERQEEKFALEQLLVDGLQGFYIAKPKPFTVKDEQSARSQTAG