Protein Info for Shew_2051 in Shewanella loihica PV-4

Annotation: acyl-CoA dehydrogenase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 758 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details transmembrane" amino acids 543 to 562 (20 residues), see Phobius details PF02771: Acyl-CoA_dh_N" amino acids 82 to 190 (109 residues), 69.9 bits, see alignment E=4.8e-23 PF02770: Acyl-CoA_dh_M" amino acids 194 to 293 (100 residues), 36 bits, see alignment E=1.3e-12 PF00441: Acyl-CoA_dh_1" amino acids 317 to 457 (141 residues), 44.7 bits, see alignment E=3.3e-15 PF09317: ACDH_C" amino acids 471 to 755 (285 residues), 368.7 bits, see alignment E=5e-114

Best Hits

Swiss-Prot: 56% identical to FADE_ECOLI: Acyl-coenzyme A dehydrogenase (fadE) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to slo:Shew_2051)

MetaCyc: 56% identical to acyl-CoA dehydrogenase (Escherichia coli K-12 substr. MG1655)
RXN-17775 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; RXN-17796 [EC: 1.3.8.8, 1.3.8.7]; 1.3.8.- [EC: 1.3.8.8, 1.3.8.7]

Predicted SEED Role

"Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2)" in subsystem Isoleucine degradation (EC 1.3.99.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.7, 1.3.99.2

Use Curated BLAST to search for 1.3.8.7 or 1.3.8.8 or 1.3.99.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QEL9 at UniProt or InterPro

Protein Sequence (758 amino acids)

>Shew_2051 acyl-CoA dehydrogenase (RefSeq) (Shewanella loihica PV-4)
MTLFILALLVIIVLFGVKNIRMQCITRPVFAFFKKVLPPLSDTEKEAMEAGDVWWEAELF
RGKPNWDTLHSYGRPRLTEEEKAFIDEQVMTALTMIDDFNIVQERKDLPPELWDYFKKEG
FFALIIPKEYGGKAFSAYANSTIVSKLASRSVSAAVSVMVPNSLGPGELLTHYGTKEQRD
HWLPKLASGEAVPCFALTGPEAGSDAGAIPDTGIVCRGEFQGEEVLGLKLNWDKRYITLA
PVSTVLGLAFQMRDPEGLLGDKEEIGITCALIPTDHPGVEIGNRHNPLNMGFMNGTTRGE
DVFIPLDWIIGGPQYAGRGWRMLVECLSAGRGISLPALATASGHVTTKTTTAYSYVRHQF
GLSLGQFEGVQEALARIIANTYQLEAARRLTTTGIDLKVKPSVVTAIAKYHMTEMGRDVL
NDAMDIQSGKGIQLGPKNYLGHGYMATPISITVEGANILTRSLMIFGQGATRCHPYVLAE
MEAAAMEDTGEALERFDNLLLGHMGYAARNALSSLWGALTGSRFNSAPVSGETKQYYKDM
TRLSAALAFVTDLSMLVMGGDLKRKEMLSARLGDVLSELYLGSATLKLFEDNGRQQDDLP
TVHYVMQLRLQRAAKALYGALRNFPNRPLAYAMRALVFPLGNHFNGPDDKLTIAVCQSML
RPGPARDRLTHLCPDFEGDNGGIAEVEDAFIAQYKAKHLFKTLRNAQKEGKLERKQANDA
LYAQAYELKIITEAEHDTLLEADRLRKIAIAVDEFESL