Protein Info for Shew_2046 in Shewanella loihica PV-4

Annotation: phosphogluconate dehydratase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 608 transmembrane" amino acids 159 to 176 (18 residues), see Phobius details TIGR01196: phosphogluconate dehydratase" amino acids 2 to 601 (600 residues), 1020 bits, see alignment E=0 PF00920: ILVD_EDD" amino acids 65 to 598 (534 residues), 524.6 bits, see alignment E=1.4e-161

Best Hits

Swiss-Prot: 66% identical to EDD_ECOLI: Phosphogluconate dehydratase (edd) from Escherichia coli (strain K12)

KEGG orthology group: K01690, phosphogluconate dehydratase [EC: 4.2.1.12] (inferred from 100% identity to slo:Shew_2046)

MetaCyc: 66% identical to phosphogluconate dehydratase (Escherichia coli K-12 substr. MG1655)
Phosphogluconate dehydratase. [EC: 4.2.1.12]

Predicted SEED Role

"Phosphogluconate dehydratase (EC 4.2.1.12)" in subsystem Entner-Doudoroff Pathway (EC 4.2.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QEL4 at UniProt or InterPro

Protein Sequence (608 amino acids)

>Shew_2046 phosphogluconate dehydratase (RefSeq) (Shewanella loihica PV-4)
MHSVVQSVTDRIIARSQATRAKYLAALGEAKANGVHRSSLSCGNLAHGFAACSPADKNAI
KAFNKANIGIVTAFNDMLSAHQPYEHYPQLLKDACHEVGSVAQVAAGVPAMCDGVTQGQP
GMELSLLSREVIAMSTAVGLSHNMFDGALLLGICDKIVPGLLIGALSFGHLPMLFVPAGP
MKSGIPNKEKARVRQKFAQGQVDRAALLEAESKSYHSAGTCTFYGTANSNQLMLEVMGLQ
LPGSSFVNPDDPLREALNKMAAKQVCRLTEMGTQYTPIGEVVNEKSVVNGIVALLATGGS
TNLTMHIVAAARAAGIIVNWDDFSELSDAVPLLARVYPNGHADINHFHAAGGMAFLIKEL
LDAGLLHEDVLTVAGPGLRRYTQEPRLIDNELVWVEGPNESLDKEVLTAVASPFQANGGL
KLLKGNLGRAVIKVSAVAESHRFVEAPAVVIDDQNKLEGLFKAGELDRDCVVVVKGQGPK
ANGMPELHKLTPILGSLQDKGFKVALLTDGRMSGASGKVPAAIHLTPEALDGGLIAKVEN
GDLIRVDANTGEVSLLVDEAVLASREVEKVDLRSTSFGMGRELFAALRSNLSSPETGARC
TTTIDENY