Protein Info for Shew_1873 in Shewanella loihica PV-4

Annotation: glucan 1,4-alpha-glucosidase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 833 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details PF09137: Glucodextran_N" amino acids 48 to 349 (302 residues), 272.5 bits, see alignment E=4.2e-85 TIGR01535: glucan 1,4-alpha-glucosidase" amino acids 92 to 829 (738 residues), 743.3 bits, see alignment E=1.3e-227 PF00723: Glyco_hydro_15" amino acids 367 to 812 (446 residues), 151.9 bits, see alignment E=2.8e-48

Best Hits

KEGG orthology group: K01178, glucoamylase [EC: 3.2.1.3] (inferred from 100% identity to slo:Shew_1873)

Predicted SEED Role

"Glucoamylase (EC 3.2.1.3)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis (EC 3.2.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QE41 at UniProt or InterPro

Protein Sequence (833 amino acids)

>Shew_1873 glucan 1,4-alpha-glucosidase (RefSeq) (Shewanella loihica PV-4)
MTSKPVPFTRTLIALSLGALLSACGGAKSDIDAAPAPSASVSETISVAPGAPGASPTWAF
SGKTGIGTSFEPYVTNAQGADQYLSSQANPVSRVWFSLAQGILTETMYGLIHNAQLKEAQ
FIISGNGFVDTEKHNTVTSIDYLHKDEAGRPLSLAYKVINKDIEGKYQIEKHFFTDPDRD
SLVMQVFFTAFEPGITPYLYVNPHIDNSGANDIASVSQDGNSLFAYTGDKDSSVLTVRGD
IPFTASSVGFVGESDGLTDLKANGELNWRYASTSADKQSLGNVAMTAALPTLEVGDANRP
SLRTTLVFGFGADKASSEQNAKDTLKDGYNKVLAAYNGEGDAIGWQDYLHSLSSLNAMTA
NTADEGKLLYTSAMVLKAQEDKTHAGALIASLSNPWGDSVSAVTGSTGYKAVWPRDFYQC
AMAFLAMGDTQTPKVAFEYLKKVQVTAATPGYSGVPGWFLQKTHVDGQIEWVGVQLDQTA
MPIMLGWKLWQAGVLSDNEIASWYQTMLKPAADFLISGGEVNLDWNYTQITPLKTQQERW
EEQQGYSPSTAAAVIAGLITASDIAKQAGDLNSSEKYLASAKSMAEKLDSLVVTQQGLLS
AEDGTKAPYYLRLAPNGTPDSADKLADNNGRAGLDQREVLDGGFLELVRYGVKSANDKTI
NDTLKLIDNTALEDNLRVRYEFTAKDGSTFPGFRRYGNDGYGEDTATGANYAESGSNTPG
QRGRVWPFFTGERAHFELAKAIADGTLNDASKAQLVQTYVKGMEAFANEGLMLPEQVWDG
VGDKTRFNYQLGQGTNSATPLAWTHAEYVKMVRSITEAKVWDHYEDVAKALNN