Protein Info for Shew_1809 in Shewanella loihica PV-4
Annotation: NAD-glutamate dehydrogenase (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00260, glutamate dehydrogenase [EC: 1.4.1.2] (inferred from 63% identity to asa:ASA_1992)Predicted SEED Role
"NAD-specific glutamate dehydrogenase (EC 1.4.1.2), large form" in subsystem Glutamate dehydrogenases or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.2)
MetaCyc Pathways
- L-alanine degradation II (to D-lactate) (3/3 steps found)
- L-glutamate degradation I (1/1 steps found)
- ethene biosynthesis IV (engineered) (1/3 steps found)
- 4-aminobutanoate degradation V (3/7 steps found)
- L-glutamate degradation V (via hydroxyglutarate) (5/10 steps found)
- L-glutamate degradation XI (reductive Stickland reaction) (2/7 steps found)
- methylaspartate cycle (9/19 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.4.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A3QDX7 at UniProt or InterPro
Protein Sequence (1614 amino acids)
>Shew_1809 NAD-glutamate dehydrogenase (RefSeq) (Shewanella loihica PV-4) MALKDAMPSVLLENVVSLIHSKVPNTQAKQVEQFATCIYAHMSKDDLQHRNDSDLYGAVL SLWNAANKTPVGETHIRVFNPSQSKHGWKSSHSIIEVIQPDMPFLVDSVGMALNRMGITT HMMLHTPLTVKRDQGVITGVSYNDDKDESNDKVAVFLIEVDRLSSDADIKSLEKEIQSVL GDVAASVNDWQAMSNKLSETIAELPSRPFPGEKRELDEAINFLTYLNNHHFTLLGYRRYD LHKVEGDLELLPANETSLGLMNVPGKPKSSKGLMLSSLSDTARKEALDSSLLILTKSTEK SRVHRPAYVDYIGIKRFDEQGNVIGEDRFIGLYASNLYNRSPREIPLLAEKIQRVLDDSG LTPHSHDYKALMHILETLPRDEIVQARESELASMAHGVLEMQDRDKLKLFVRKDGFGRFL SCLVYVSKDRYNTKLREDTQRILAQHFNTKEDVEFTTYFSESTLARTHYIVKVDNNITDV DVAAIENNLIEAARSWEDKLNTALITAQGEESGNRLVKRYVNAFPRSYKEDVLPSSAVVD IEHLEALDDDHKLGMLFYQPQETALKDNKVRLKLFHKDEPIHLSDVLPMLENFGLRVINE RPYEVMTADGSTFWILDFLMTVQGAAVDNLADSQDRFQTALSQVWRKELEDDGFNRLVLA TGLSGREVSVLRAYAKYMRQIDATFSQAYIEETFSSYPQIADLLVKMFIRKFNPKLKTRT LAKFVEQIDMRLEDVSSLDDDRIIRRYLDLINATLRTNFYQVLPDGSNKPYVSFKFSPEE IPEMPRPLPKFEIFVYSPRVEGVHLRGGKVARGGLRWSDRREDFRTEVLGLVKAQQVKNT VIVPVGAKGGFVCKQLPTEGGRDAFFTEGQECYRLFIRGLLDISDNIVEGEIVPPANVVR HDEDDPYLVVAADKGTATFSDIANAISEEYGFWLGDAFASGGSNGYDHKKMGITARGAWE SVKRHFREIGVDCQTTDFTCLAIGDMAGDVFGNGMLLSEHTRLVTAFNHMHIFIDPNPDA ASSYKERARLFEMPRSSWEDYNKDLISKGGGIFLRSAKSITLTPEMKKMLDTKKASMTPN ELLKELLKMKVDLIWNGGIGTYIKATSETHAEVGDRANDALRVNGNEVQARIIGEGGNLG CTQLGRIEYAANGGRMNTDFVDNVGGVDCSDNEVNIKILLNAMVAEGEMTVKQRNRLLVE MTDEVSRIVLQDCKDQTRTISVTQVRGAEQLKEQIRFIHYLEKEDKLDRALEFLPTDDEL AERLANGKSLTRPELSVLVAYAKMVLKEQLLTPEITDDSFLSQLLIEYFPRQLQEKYSER MVTHPLRAEIIATSLANELVNDMGLNFVQRMQDETGASVAEAAICYTMAREVFGLAELTK SITALNGVVPAVVQGEMLHQLRRNLRRACRWFLRHRNRNQSIEQVVAFFSPVFQELRQNV HQYLVEDEVNGIRAEIAALVKEGVPEQVATDVVNMSTLFSALDISQIAELEDKPVALVAE TYYKLGARIDLHWFLEQISAQPVANHWQALARAAFREELDWQQRSLSSVVLRTCPSVCEA DAIIDQWVESNQGLLERWFHMLADFKTSQTHEFAKFSVALRELNLLILHCEGQS