Protein Info for Shew_1301 in Shewanella loihica PV-4

Annotation: phosphoribosylformylglycinamidine synthase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1293 TIGR01735: phosphoribosylformylglycinamidine synthase" amino acids 4 to 1290 (1287 residues), 2069 bits, see alignment E=0 PF18076: FGAR-AT_N" amino acids 35 to 149 (115 residues), 149.9 bits, see alignment E=7.3e-48 PF18072: FGAR-AT_linker" amino acids 170 to 219 (50 residues), 81.9 bits, see alignment (E = 9.4e-27) PF02769: AIRS_C" amino acids 430 to 587 (158 residues), 125.1 bits, see alignment E=7.2e-40 amino acids 836 to 964 (129 residues), 52 bits, see alignment E=2.3e-17 PF22689: FGAR-AT_PurM_N-like" amino acids 646 to 804 (159 residues), 277.7 bits, see alignment E=6.5e-87 PF13507: GATase_5" amino acids 1038 to 1289 (252 residues), 367.8 bits, see alignment E=6e-114

Best Hits

Swiss-Prot: 72% identical to PUR4_IDILO: Phosphoribosylformylglycinamidine synthase (purL) from Idiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR)

MetaCyc: 70% identical to phosphoribosylformylglycinamide synthetase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylformylglycinamidine synthase. [EC: 6.3.5.3]

Predicted SEED Role

"Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)" in subsystem De Novo Purine Biosynthesis (EC 6.3.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QCH3 at UniProt or InterPro

Protein Sequence (1293 amino acids)

>Shew_1301 phosphoribosylformylglycinamidine synthase (RefSeq) (Shewanella loihica PV-4)
MEIIRGAPALSAFRVQKLMEACQSAALPVQDIYAEFIHLADLTEALDAGETQQLAKLLTY
GPAIEAHAPQGNLYFVTPRPGTISPWSSKATDIAHNCGLNKVKRLERGIAYYVQADNLDH
AQQKQLLGLLHDRMVEVVLPDFEQASSLFARTEPAVFTSVDVLGEGKAALEAANVKLGLA
LAPDEIDYLVENFQRLNRNPNDVELMMFAQANSEHCRHKIFNADWTIDGEVQPKSLFKMI
KNTFEKTPDHVLSAYKDNAAVMEGSVAGRFFPEPNGVYAYHTEPMHILMKVETHNHPTAI
SPYPGAATGSGGEIRDEGATGRGSKPKAGLTGFSVSNLKIPGFVQPWEGDYGKPDRIVTA
LDIMTEGPLGGAAFNNEFGRPALLGYFRTYEQEVSSHNGVEVRGYHKPIMLAGGLGNIRE
EHVQKGEITVGAKLIVLGGPAMNIGLGGGAASSMASGQSSEDLDFASVQRDNPEMERRCQ
EVIDRCWQMGEANPIQFIHDVGAGGLSNAFPELVDDGGRGGRFELRNVPSDEPGMSPLEI
WCNESQERYVLSVAPENLELFTAICERERAPFAVVGEATEERHLTLSDEHFDNKPIDLPL
EVLLGKAPKMSRDVVSQKADSPALAQETIELKDAVRRVLRLPTVAEKTFLITIGDRTVTG
LVNRDQMVGPWQVPVADCAVTASSFDSYSGEAMSMGERTPLALLDFGASARMAVAESIMN
IAGSDIGSLKRIKLSANWMSAAGHPGEDAGLYEAVKAVGEELCPELDLTIPVGKDSMSMK
TAWQDGGEDKTVTSPMSLVITAFGAVQDIRKTVTPELRSDKGDTELLLVDLSQGERRLGG
SCLAQVYGELGDKAPDLADAALLRGFFEVTQALVADKALLAYHDRSDGGLFTTLVEMAFA
GNTGLDVDLSALTGTHLERLFNEELGAVIQVRAADSQAIKAQYQAAGVACRVVAKPVAGD
SIIIRDAGAEVLAESRTELRTIWAETTYRMQAMRDNPECAEEEFKLKQVADAPGLTVDLK
FDPSEDIAAPYILKGTAPKMAILREQGVNSHLEMAAAFDRAGFESRDVHMSDILSGRISL
EEFQGLAACGGFSYGDVLGAGEGWAKSILFNSRAREEFSRFFERNDSFALGVCNGCQMLS
NLKDIIPGTEHWPHFVRNRSERFEARFSLVEVQKSPSFFFEGMEGSRMPIAVSHGEGRAE
FASPEALAAAEASGTIALRFVDGHGQVATQYPENPNGSPNALTGICSTDGRVTIMMPHPE
RVFRTVANSWHPDEWGEDSPWMRMFRNVRAKLG