Protein Info for Shew_1260 in Shewanella loihica PV-4

Annotation: DNA methylase N-4/N-6 domain-containing protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 719 PF01555: N6_N4_Mtase" amino acids 160 to 520 (361 residues), 68.8 bits, see alignment E=3.4e-23

Best Hits

KEGG orthology group: K00571, site-specific DNA-methyltransferase (adenine-specific) [EC: 2.1.1.72] (inferred from 100% identity to slo:Shew_1260)

Predicted SEED Role

"Type III restriction-modification system methylation subunit (EC 2.1.1.72)" in subsystem Restriction-Modification System (EC 2.1.1.72)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.72

Use Curated BLAST to search for 2.1.1.72

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QCD2 at UniProt or InterPro

Protein Sequence (719 amino acids)

>Shew_1260 DNA methylase N-4/N-6 domain-containing protein (RefSeq) (Shewanella loihica PV-4)
MSRLTDLLAKAKAKDSQLGADLEREFKVLSSRLSFGLNFERHSPEAVELPLRSIRKGDKV
RVLPERGSAKKGDQRLWQVKAIHKAQNIADLELLDTAEPEMQSAPLSELIVVAEFRDTIY
PGLVSTGKVTRGGDKPFHSVINGENYHVLKALTYTHRGKIDAIYIDPPYNSGAKDWKYNN
DYVEGDDLYRHSKWLAMMERRLFVAKELLNPADSVLIVTIDEKEYLRLGLLLEQVFPEAK
IQMVSSVINPFGVARAGQFARVDEYIFIVNFGQSVPVQTPIQTLQVGDSMQVNGDEDLAE
QAGENLKKVDKTTYWFSFMRAGSNSSRSHSPGCFYPIFVSKDGAIKAIGHPLPKGESKDN
VKTPAGTYAAWPVNEAGGEAVWQASHENAKQLLASGYIRSGRFNKKLGRHSITYLREGTV
EAIANGLLTIADGEINRVVEQTRTYVGKSTWNIRSHNATSQGTKLLGRILPRRKFPYPKS
LYAVEDALRFFVSDKPDAIILDFFSGSGTTAHAVMRLNRQDGGRRQCISVTNNEVAADEQ
KALREQGLRPGDAEWEKYGICDYITKPRVAAAITGKTPSGDPIKGDYKFTDEFPMADGFE
ENAEFFTQTYEAKNAVNHNLAYARIAPLLWLRAGAKGSRIDELPVEGWAVADTYGLLNEV
DEATPFIDAVGAVNGLRIAYIVTDDDRRFQAIAKRLPEGVEPVRLYESYLSNFSFANGE