Protein Info for Shew_1230 in Shewanella loihica PV-4

Annotation: phosphoribosylaminoimidazole carboxylase, catalytic subunit (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 52% identical to PURE_THEMA: N5-carboxyaminoimidazole ribonucleotide mutase (purE) from Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)

KEGG orthology group: K01588, 5-(carboxyamino)imidazole ribonucleotide mutase [EC: 5.4.99.18] (inferred from 100% identity to slo:Shew_1230)

MetaCyc: 45% identical to N5-carboxyaminoimidazole ribonucleotide mutase (Escherichia coli K-12 substr. MG1655)
5-(carboxyamino)imidazole ribonucleotide mutase. [EC: 5.4.99.18]

Predicted SEED Role

"Phosphoribosylaminoimidazole carboxylase catalytic subunit (EC 4.1.1.21)" in subsystem De Novo Purine Biosynthesis (EC 4.1.1.21)

MetaCyc Pathways

superpathway of histidine, purine, and pyrimidine biosynthesis (45/46 steps found)
superpathway of purine nucleotides de novo biosynthesis II (25/26 steps found)
superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
inosine-5'-phosphate biosynthesis I (6/6 steps found)
inosine-5'-phosphate biosynthesis II (5/5 steps found)
inosine-5'-phosphate biosynthesis III (5/6 steps found)

KEGG Metabolic Maps

Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.21, 5.4.99.18

Use Curated BLAST to search for 4.1.1.21 or 5.4.99.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QCA2 at UniProt or InterPro

Protein Sequence (169 amino acids)

>Shew_1230 phosphoribosylaminoimidazole carboxylase, catalytic subunit (RefSeq) (Shewanella loihica PV-4)
MTKPFVAVLMGSDSDLPTMQATLDVLKAFGIQYEAKVTSAHRTPAATHAYVHDAEERGCK
VFICAAGLAAHLAGAVAGITTRPVIGVPVDCGPLQGHDALLSTVMMPGGVPVATVAIGSA
GAKNAGYLAAQMLAVGDDALALAVKEERAKSAEAVQAKDAKLQEKLANA