Protein Info for Shew_1169 in Shewanella loihica PV-4

Annotation: glycogen debranching enzyme GlgX (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 733 TIGR02100: glycogen debranching enzyme GlgX" amino acids 6 to 709 (704 residues), 1005.2 bits, see alignment E=6.4e-307 PF02922: CBM_48" amino acids 10 to 97 (88 residues), 60.4 bits, see alignment E=1.8e-20 PF00128: Alpha-amylase" amino acids 189 to 367 (179 residues), 39.3 bits, see alignment E=5e-14

Best Hits

KEGG orthology group: K02438, glycogen operon protein GlgX [EC: 3.2.1.-] (inferred from 100% identity to slo:Shew_1169)

Predicted SEED Role

"Glycogen debranching enzyme (EC 3.2.1.-)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 3.2.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QC41 at UniProt or InterPro

Protein Sequence (733 amino acids)

>Shew_1169 glycogen debranching enzyme GlgX (RefSeq) (Shewanella loihica PV-4)
MRLTHGKPYPLGATLDSEGVNFALFSAHATAVYLCLFDEEDREVARLRLERQSQQIWHGH
VSGLRAGCRYGYRVDGPYQPLIGHRFNVNKLLLDPYARRLSGAIKDHKSHYGYQFGHEQE
DLSFCELDNAERMPKCVVVDLENLKPITPLVNPFDQSDSPLSLRRSVIYELHVKGFSQQN
PEVASERRGRFAGLGDPASLQHLKSLGVTAVELLPVQQFVTEAFLQQKGLTNYWGYNTIG
YFAPHQAYLSESISESEGDGAIEEFRDMVSALHGAGIEVILDVVYNHSAEGNRLGPTYSF
KGIDNLSYYRLHPSEPRYYINDTGCGNTLNLSNPRVLQLVMDSLRYWVEVMGVDGFRFDL
ASTLGREDHGFDVGSGFFDAISQDPVLSRVRLIAEPWDIGPGGYQLGQFPISWSEWNDRY
RDTVRRFWRGDAGMLPEFATRFHGSSDLFEHAARKPAASINFITSHDGFTLMDLVSYRHK
HNQANGEQNRDGHNENYSDNYGHEGQSDELALTALRARQCRNMLTTLFMSQGVPMLLAGD
EVGHSQLGNNNAYCQDNPASWLDWRKDIDSDELLSFTANLIKLRRRFPMLCHRDYIHESA
NQASPGLDWFCRQGEPMTKAHWGEAQTRTLSLVISGELEAQAGCRQALLLMFNADNRPQQ
FTLPSLEGIVQWQCLLSTQDSPQYGESASYQALLQGASLLLQDRSLMIYYAQFTSAQEEP
SAREMPSAKGESR