Protein Info for Shew_1157 in Shewanella loihica PV-4

Annotation: alkaline phosphatase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 545 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF00245: Alk_phosphatase" amino acids 76 to 533 (458 residues), 437.9 bits, see alignment E=2.2e-135

Best Hits

KEGG orthology group: K01077, alkaline phosphatase [EC: 3.1.3.1] (inferred from 100% identity to slo:Shew_1157)

Predicted SEED Role

"Alkaline phosphatase (EC 3.1.3.1)" in subsystem Phosphate metabolism (EC 3.1.3.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.1

Use Curated BLAST to search for 3.1.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QC29 at UniProt or InterPro

Protein Sequence (545 amino acids)

>Shew_1157 alkaline phosphatase (RefSeq) (Shewanella loihica PV-4)
MNKKLLVLSLAAILGLAACGSDGDNGKDGTAGTPGTDGSNGTNGKDWTGVNQWYVDGQNR
VTKASGMTNNNEAGAAKNIILFVGDGMGVSTVTAARILDGQLKGQTGEENSLSFETLPHL
GLAKTYNVDGQTPDSAGTMTAMVTGVKTDVGVISQGEGVERGVCSSTTGQDLVTSLELAA
MAGMSTGVVSTARITHATPAATYAHVPERDWEADSNLPAEAVTNGCKDIAAQMLDFNDGK
GINVVMGGGRRAFIPNTTVDPEGKAGKRKDGRDLTAEWMAQYPNAAYVTDRDSFLAMDTS
TTEHALGLFNSSHMEYDYDRVEDGVNGEPSLAEMTAKSIDILSKNDKGFVLIVEAGRIDH
AHHAGNAARALHDTIALSEAVRVAMEKTSANDTLLMVTADHSHVFTIAGYPTRGNPILGL
VKSNDSNGQPSVTNSTDANGMPYTTVGYANGLGFGSLETGGDERYNYAAEPGRVDLNYID
TQSAGFHQEALVPLESETHAGEDVAIFARGPGASLVEGTVEQNHIFHVMNYAGDLVKKAE
AAAAQ