Protein Info for Shew_1132 in Shewanella loihica PV-4

Annotation: RdgB/HAM1 family non-canonical purine NTP pyrophosphatase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 73% identical to IXTPA_SHEON: dITP/XTP pyrophosphatase (SO_3358) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K01516, nucleoside-triphosphatase [EC: 3.6.1.15] (inferred from 100% identity to slo:Shew_1132)

MetaCyc: 56% identical to dITP/XTP pyrophosphatase (Escherichia coli K-12 substr. MG1655)
Nucleotide diphosphatase. [EC: 3.6.1.66, 3.6.1.9]; 3.6.1.66 [EC: 3.6.1.66, 3.6.1.9]; 3.6.1.66 [EC: 3.6.1.66, 3.6.1.9]

Predicted SEED Role

"Nucleoside 5-triphosphatase RdgB (dHAPTP, dITP, XTP-specific) (EC 3.6.1.15)" in subsystem Heat shock dnaK gene cluster extended (EC 3.6.1.15)

MetaCyc Pathways

superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
pyrimidine deoxyribonucleotides de novo biosynthesis II (7/7 steps found)
pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
pyrimidine deoxyribonucleotides biosynthesis from CTP (7/8 steps found)
pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
UTP and CTP dephosphorylation I (5/7 steps found)
dZTP biosynthesis (3/5 steps found)
pyrimidine deoxyribonucleotides dephosphorylation (1/3 steps found)
tunicamycin biosynthesis (2/9 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.66 or 3.6.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QC05 at UniProt or InterPro

Protein Sequence (223 amino acids)

>Shew_1132 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase (RefSeq) (Shewanella loihica PV-4)
MPYRFPASLGMIAAIETCHHESAKMNQIVLASGNKGKLKEFAEILAEFGIEVLPQSQFNV
QEVPETGTTFVENAIIKARHAAQVTGLAAIADDSGLEVDALEGAPGIYSARYGGEGASDS
DNYTKLLGALNDNDKRSARFQCILVYMRHAKDPTPIICQASWEGSIGFEPKGDNGHGYDP
VFIPMDHECSAAELRSDEKNALSHRGKAMQLLLSELKAKGVLG