Protein Info for Shew_1052 in Shewanella loihica PV-4

Annotation: GTP-binding protein LepA (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01393: elongation factor 4
  • amino acids 2 to 595 (594 residues), 992.4 bits, E=6.6e-303
• PF00009: GTP_EFTU
  • amino acids 2 to 182 (181 residues), 188.8 bits, E=1.5e-59
• TIGR00231: small GTP-binding protein domain
  • amino acids 6 to 176 (171 residues), 62.2 bits, E=5.2e-21
• PF03144: GTP_EFTU_D2
  • amino acids 205 to 275 (71 residues), 34.1 bits, E=6.1e-12
• PF00679: EFG_C
  • amino acids 399 to 483 (85 residues), 71.2 bits, E=1.2e-23
• PF06421: LepA_C
  • amino acids 487 to 593 (107 residues), 162.9 bits, E=4.7e-52

Best Hits

Swiss-Prot: 100% identical to LEPA_SHELP: Elongation factor 4 (lepA) from Shewanella loihica (strain ATCC BAA-1088 / PV-4)

KEGG orthology group: K03596, GTP-binding protein LepA (inferred from 100% identity to slo:Shew_1052)

MetaCyc: 82% identical to 30S ribosomal subunit biogenesis factor LepA (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"Translation elongation factor LepA" in subsystem Heat shock dnaK gene cluster extended or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (7/8 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See A3QBS5 at UniProt or InterPro

Protein Sequence (596 amino acids)

>Shew_1052 GTP-binding protein LepA (RefSeq) (Shewanella loihica PV-4)
MKHIRNFSIIAHIDHGKSTLSDRLIQECGGLSDREMAAQVLDSMDLERERGITIKAQSVT
LDYKAKDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAL
DMDLDVVPVLNKIDLPQADPERVAEEIEDIVGIEAADAVRCSAKTGLGISDVLETIVAQI
PPPEGDPEAPLQALIIDSWFDSYLGVVSLVRIKHGVLKKGDKFKVMSTGQTHTADRVGIF
TPKQTDTAQLSTGEVGFVIAGIKEIHGAPVGDTLTLAKNGADKPLPGFKKVKPQVYAGVF
PISTDDYENFRDALNKLSLNDASLFFEPETSSALGFGFRIGYLGLLHMEIIQERLEREYN
LDLITTAPTVVYEVVTTKGETIYVDNPSDLPPMNNIEEMREPIVETNILVPKEYLGNVIT
LCIEKRGVQTNMVYHGNQVAITYDMPMAEVVMDFFDRLKSTSRGYASLEYNFKRFEAADM
VRLDVLINGDRVDALAMILHKANVRYQGLALVNKMKELIPRQMFDIAIQAAVGSQIIARS
SVKALRKDVTAKCYGGDVSRKKKLLQKQKEGKKRMKQVGNVEVPQEAFLAVLKINE