Protein Info for Shew_0975 in Shewanella loihica PV-4

Annotation: binding-protein-dependent transport systems inner membrane component (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 301 transmembrane" amino acids 21 to 40 (20 residues), see Phobius details amino acids 83 to 106 (24 residues), see Phobius details amino acids 118 to 139 (22 residues), see Phobius details amino acids 169 to 189 (21 residues), see Phobius details amino acids 223 to 242 (20 residues), see Phobius details amino acids 268 to 289 (22 residues), see Phobius details PF00528: BPD_transp_1" amino acids 117 to 298 (182 residues), 59.6 bits, see alignment E=1.8e-20

Best Hits

Swiss-Prot: 62% identical to POTH_ECOLI: Putrescine transport system permease protein PotH (potH) from Escherichia coli (strain K12)

KEGG orthology group: K11075, putrescine transport system permease protein (inferred from 100% identity to slo:Shew_0975)

MetaCyc: 62% identical to putrescine ABC transporter membrane subunit PotH (Escherichia coli K-12 substr. MG1655)
ABC-25-RXN [EC: 7.6.2.11, 7.6.2.16]

Predicted SEED Role

"Putrescine transport system permease protein PotH (TC 3.A.1.11.2)" in subsystem Polyamine Metabolism (TC 3.A.1.11.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.11 or 7.6.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QBJ8 at UniProt or InterPro

Protein Sequence (301 amino acids)

>Shew_0975 binding-protein-dependent transport systems inner membrane component (RefSeq) (Shewanella loihica PV-4)
MKKSLKFRLPKGQCWTIGVPYFWLLMFFALPFAIVLKISLSTPAVAIPPYEALYQYADES
LQIMLNLGNYILIFEDHLYINAYLGSLKMACVTTIGCLLIGYPMAYAIARAPKQHQTVLI
LLVMLPSWTSFLIRVYAWMGILSNNGVINNLLMWLGVISEPIQMLNTNFAVYIGIIYTYL
PFMILPLYANLSQLDGSLLEAAADLGSRSLNTFWKVTLPLSKGGVIAGSMLVFIPVVGEF
VIPELLGGPDSLMIGKVLWQEFFNNRDWPVASSLAIVMLALLIIPITLFHRYQSRSMEKT
I