Protein Info for Shew_0923 in Shewanella loihica PV-4

Name: valS
Annotation: valyl-tRNA synthetase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 959 transmembrane" amino acids 504 to 524 (21 residues), see Phobius details TIGR00422: valine--tRNA ligase" amino acids 1 to 937 (937 residues), 1248.9 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 14 to 631 (618 residues), 527.7 bits, see alignment E=4.9e-162 PF09334: tRNA-synt_1g" amino acids 40 to 189 (150 residues), 29.3 bits, see alignment E=9.9e-11 PF13603: tRNA-synt_1_2" amino acids 246 to 347 (102 residues), 39.7 bits, see alignment E=9.5e-14 PF08264: Anticodon_1" amino acids 680 to 830 (151 residues), 145.7 bits, see alignment E=2.5e-46 PF10458: Val_tRNA-synt_C" amino acids 890 to 954 (65 residues), 66.8 bits, see alignment 4.1e-22

Best Hits

Swiss-Prot: 72% identical to SYV_ECOLI: Valine--tRNA ligase (valS) from Escherichia coli (strain K12)

KEGG orthology group: K01873, valyl-tRNA synthetase [EC: 6.1.1.9] (inferred from 72% identity to aha:AHA_3702)

MetaCyc: 72% identical to valine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Valine--tRNA ligase. [EC: 6.1.1.9]; 6.1.1.9 [EC: 6.1.1.9]; 3.1.1.- [EC: 6.1.1.9]; RXN-23924 [EC: 6.1.1.9]

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QBE6 at UniProt or InterPro

Protein Sequence (959 amino acids)

>Shew_0923 valyl-tRNA synthetase (RefSeq) (Shewanella loihica PV-4)
MEKTYNPQSIEQALYQNWEEKGYFKPHGDESNGNYCIMIPPPNVTGSLHMGHAFQDTIMD
TLIRYQRMKGKNTLWQVGTDHAGIATQMLVERKVEAEEGKSRHDLGRETFIDRIWDWKNQ
SGGTITKQLRRLGASVDWDRERFTMDEGMSAAVQEVFVRLYNDDLIYRGKRLVNWDPKLH
TAISDLEVENKEKQGSMWHFRYPLADGALTADGKDYLEVATTRPETMLGDSAVAVHPDDE
RYQSLIGKFILLPIVNRRIPIVADDYVDMEFGTGCVKITPAHDFNDYEVGKRHNLPMFNI
LTIDAAIRSQAEVVNSDGTANDELDGSLPERFAGLDRFKARTAIVDEFESLGLLGKIDPH
ALKVPYGDRSGVVIEPLLTDQWYVAVAPMAKTAIEAVENGDIKFVPQQYENMYFSWMRDI
QDWCISRQLWWGHRIPAWYDEAGKVYVGRDEAEVRAKHNLDDSVVLRQDPDVLDTWFSSA
LWTFSTLGWPDDTEALKTFHPTDVLVTGFDIIFFWVARMIMMTMHFIKDEDGKPQVPFKT
VYVTGLIRDEQGNKMSKSKGNVLDPLDMIDGIDLEALVEKRTGNMMQPQLAAKIEKSTRK
EFADGIEAHGTDALRFTLAAMASTGRDINWDMKRLDGYRSFCNKIWNASRYVLMNTEEQD
CGPQSPNGKADGEMQLSLADRWIVGLFNQTVKAFDEHMENYRFDLAANTLYEFTWNQFCD
WYLELTKPVLQNGTEAEQRGTRHTLVTVLEAMQRLLHPMMPYLTETIWQRVKPLAGVEGD
TLMLAEFPVYQASKVDEAAMADLEWVKQVIVAVRNIRAELNIAPSKPLNAMLRSVSAQDK
ARVEANQTFFATLAKLESMTILADGDTAPMSTTQLVGEMELLIPMAGLIDVAAEMARIDK
QFEKLVGEAKRIEGKLNNQGFVAKAPEAVIEKERAKLAEFQRDMDKLCEQKAELAKLEG