Protein Info for Shew_0813 in Shewanella loihica PV-4

Annotation: diguanylate cyclase/phosphodiesterase with PAS/PAC and GAF sensor(s) (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 837 TIGR00229: PAS domain S-box protein" amino acids 168 to 287 (120 residues), 40.8 bits, see alignment E=2.2e-14 amino acids 285 to 409 (125 residues), 75 bits, see alignment E=5.6e-25 PF08448: PAS_4" amino acids 172 to 280 (109 residues), 37.8 bits, see alignment E=7.2e-13 amino acids 294 to 403 (110 residues), 24.5 bits, see alignment E=9.6e-09 PF13426: PAS_9" amino acids 177 to 278 (102 residues), 20.2 bits, see alignment E=2.1e-07 amino acids 299 to 400 (102 residues), 56.3 bits, see alignment E=1.2e-18 PF13188: PAS_8" amino acids 292 to 339 (48 residues), 20.5 bits, see alignment 1.3e-07 PF00989: PAS" amino acids 292 to 399 (108 residues), 51.7 bits, see alignment E=2.8e-17 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 410 to 573 (164 residues), 143.5 bits, see alignment E=5.3e-46 PF00990: GGDEF" amino acids 414 to 571 (158 residues), 152.7 bits, see alignment E=2.7e-48 PF00563: EAL" amino acids 592 to 826 (235 residues), 240 bits, see alignment E=8.2e-75

Best Hits

KEGG orthology group: None (inferred from 100% identity to slo:Shew_0813)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QB37 at UniProt or InterPro

Protein Sequence (837 amino acids)

>Shew_0813 diguanylate cyclase/phosphodiesterase with PAS/PAC and GAF sensor(s) (RefSeq) (Shewanella loihica PV-4)
MTLGRRSRSRIVIDIKIPDEALQDWQEALETLAQAFDGSLFLLALTASDGLTPQLSLCDD
NQVHRQSVAQLCAQGLSLFRDYIEHPHHPREPVDVGGIILHPLFWPSQALYGALCLVPNE
QANHNKRLNTQVKLTCQAMQYALINLYQTHRQQLIEIAPQAAPNVDLQLFIDSLQEHVWM
KDVAGRYVLINHSVEIAWGRSKEQLLNRTDDEIFSPELAEEFVQGDYESIVRGVQVTAAE
CEAKDIDQSNSWLETTKVPVVSPDGTLEGVIGISRNITSHKAAQEQLELAANIFENSVEG
VVITDASGNIIEVHGAFTEITGYSRDEVLGENPRILNSGRHDKAFFERLWRSLLTNGKWH
GEIWNRRKSGAIYPEQLTISSMYGDDHQVRYFVAVFADISAQKQSEKELARLVYHDPLTK
LPNRMMLSANLEQELRHAAREETELALIFIDVDLFKHINDSYGHVTGDAILVEVARRLGQ
KLGEEATLARLGSDEFIAVLPHVESDDSVSLMVSRLREAFDAPFLLDDEGSVRLTASMGV
AIYPGDGESGDTLLRNADTAMHRAKANGRNNYAFYTQSLTLASVEHLKLQSALHDAINKD
AFYLVYQPKLALGTGQCVGFEALLRWEDPQLGTISPAIFIPVAENIGLINEIGLWVLKQA
ATQGAQWLAEGKRFGRISVNVAGPQLQQKQFCAQVKRVLDEAGLPPEYLELEVTESCMMS
DPLAVIEVLKQLGELGVELSVDDFGTGYSSLNYLKRLPIHKLKIDQSFIRDIPRDGNNTA
IAKAVIALGHALNLCIIAEGVETPEQAAFLEAAGCDQAQGYLYSKPRMVEELAEFLG