Protein Info for Shew_0665 in Shewanella loihica PV-4

Annotation: sulfite reductase (NADPH) flavoprotein, alpha chain (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 594 TIGR01931: sulfite reductase [NADPH] flavoprotein, alpha-component" amino acids 10 to 594 (585 residues), 869.8 bits, see alignment E=5.3e-266 PF00258: Flavodoxin_1" amino acids 63 to 191 (129 residues), 116.2 bits, see alignment E=2.1e-37 PF00667: FAD_binding_1" amino acids 227 to 419 (193 residues), 92.5 bits, see alignment E=4.5e-30 PF00175: NAD_binding_1" amino acids 452 to 558 (107 residues), 65.3 bits, see alignment E=1.1e-21

Best Hits

Swiss-Prot: 76% identical to CYSJ_SHESA: Sulfite reductase [NADPH] flavoprotein alpha-component (cysJ) from Shewanella sp. (strain ANA-3)

KEGG orthology group: K00380, sulfite reductase (NADPH) flavoprotein alpha-component [EC: 1.8.1.2] (inferred from 100% identity to slo:Shew_0665)

MetaCyc: 52% identical to sulfite reductase, flavoprotein subunit (Escherichia coli K-12 substr. MG1655)
Sulfite reductase (NADPH). [EC: 1.8.1.2]; Flavin reductase. [EC: 1.8.1.2, 1.5.1.30]

Predicted SEED Role

"Sulfite reductase [NADPH] flavoprotein alpha-component (EC 1.8.1.2)" in subsystem Cysteine Biosynthesis (EC 1.8.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.2

Use Curated BLAST to search for 1.5.1.30 or 1.8.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QAN9 at UniProt or InterPro

Protein Sequence (594 amino acids)

>Shew_0665 sulfite reductase (NADPH) flavoprotein, alpha chain (RefSeq) (Shewanella loihica PV-4)
MLLKELSSLASPLSATQVDKLKQLTSELNAVQLAWVGGYLSATAELSGVAGEVQQAPQQT
ITILYGSQTGNGRGIATELAEKAQAQGYSVNLVSMADYKTRQLKQETLLLAVVSTHGEGE
APDDAIELHKFLASKRAPQLDTLNYAVLALGDSSYEFFCQTGKDFDARLSALGAKAIVPL
TECDVDYESAASQWQEAVLCAVKPLVGTGASVVSIGSASSKVSSSAFSKQKPYSAELLVS
QKLTGRDSDRDVRHVEIDLGESGLSYQVGDALGVWFSNSDALVEEIINGLSLDGEAEVTL
GGATLTLAQALKEKRELTQLYPGLVQNWAQLSGNEALQAISEDREQTRQFILKHQLADLV
AKYPVDIGAFQLVELLRPITPRLYSIASSQSEVESEVHLTVALVADERDSGTRFGGASHF
LASAEEGAEVKVYVEPNNHFRLPENPDTPVVMIGPGTGVAPFRAFMQERAAQGAQGDSWL
FFGNPHFEQDFLYQTEWQQYLKDGVLTRLDSAFSRDQAHKIYVQHRILEQGETLWQWIER
GAHLYICGDADRMAKDVHQALIEVIKQHGGKSEEQAEALLESLRSDKRYQKDVY