Protein Info for Shew_0551 in Shewanella loihica PV-4

Annotation: DNA topoisomerase IV subunit A (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 755 TIGR01062: DNA topoisomerase IV, A subunit" amino acids 12 to 746 (735 residues), 1164.8 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 36 to 473 (438 residues), 455.7 bits, see alignment E=8.2e-141

Best Hits

Swiss-Prot: 69% identical to PARC_PSEAE: DNA topoisomerase 4 subunit A (parC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02621, topoisomerase IV subunit A [EC: 5.99.1.-] (inferred from 100% identity to slo:Shew_0551)

Predicted SEED Role

"Topoisomerase IV subunit A (EC 5.99.1.-)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.-)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.-

Use Curated BLAST to search for 5.99.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QAC5 at UniProt or InterPro

Protein Sequence (755 amino acids)

>Shew_0551 DNA topoisomerase IV subunit A (RefSeq) (Shewanella loihica PV-4)
MSDAIDLSLDGVEQMPLRRFTEEAYLNYSMYVIMDRALPHIGDGLKPVQRRIVYAMSELG
LSAQSKHKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDGQGNWGAPDDPK
SFAAMRYTEARLSKFSEVLLTELGQGTVDWGVNFDGTLKEPMVLPARLPHILLNGITGIA
VGMATDVPPHNARELVDACVELIDNPKVDLERLMELVPGPDYPTEAEIISPRTEIAKIYE
TGRGSIKARAVYSVESGEIVITALPHQASGGKILEQIAAQMQAKKLPMVADLRDESDHES
PVRIVIVPRSNRVDCDQLMAHLFATTDLEKNFRVNLNVLGLNGRPQVKGLKQLLTEWLEF
RLTTVRRRLEHRLDKVLARLHILEALMIAFLNIDEVIEIIRYHDDPKAELMSRFNLTDEQ
ANAILDLKLRHLAKLEEFKIKTEQSELEAEREKLELLLSSDRRMKTLIKKELKQDAETYG
DERRSPLVERSESRALSEQELVPAEAVTVVLSEKGWVRCAKGHDIDPTALSYKAGDGFLC
AASGRSNHATVFIGSTGRAFATDTHTLPSARSQGEPVTTRFQLSPGESMAHALLGEDGQC
FLLASDAGYGFIGDYKDMVSRNKAGKALLSLPANAQVLPPKRVDKSRSESILAITNEGRM
LLFSVDALPQLAKGKGNKIIGIASDRAKSREELLVHLHVVPQDTAVTLWAGKRKLTLKPS
DLEHYRGERGRRGAKLPRGLQRVDKVELGEDETSL