Protein Info for Shew_0436 in Shewanella loihica PV-4

Annotation: diguanylate cyclase/phosphodiesterase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 590 PF00563: EAL" amino acids 10 to 239 (230 residues), 183.3 bits, see alignment E=7.8e-58 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 413 to 486 (74 residues), 50.4 bits, see alignment E=1.1e-17 PF00990: GGDEF" amino acids 413 to 483 (71 residues), 50.4 bits, see alignment E=3.3e-17

Best Hits

KEGG orthology group: None (inferred from 100% identity to slo:Shew_0436)

Predicted SEED Role

"EAL domain/GGDEF domain protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QA10 at UniProt or InterPro

Protein Sequence (590 amino acids)

>Shew_0436 diguanylate cyclase/phosphodiesterase (RefSeq) (Shewanella loihica PV-4)
MTAIDLSSELDKIIENEKIHVLFQPIFNINSQRLHGFEALSRGPEHSALFSPVPLFQTAA
HEGRLSELEEICRRHSIRQFDARELPGKLFINISPKALLEPSHAKGLTLSLVQELGLSPD
QIVIELSEQYPADDIDLLKECLNHYRSQGFLTAIDDLGTGYSGLRLWSELAPDYVKIDRH
FIHQIDQSPVKQEFVRSIIDLCQSLSCKVIAEGIETPAELALLTQLGIVYCQGYLLGRPQ
TQPTQTLKAVAPQETKQRQTRYAESAESLSTRTICVTPETKLKALSARFVRSPHLQVVAI
VDAKQKPLGVVDRAQLMELFSTPYGRALHENSPVIEVMNDKVLKVPACMPISEVSQCLTA
EHGTVAQHFMVMREQQLLGIGHTKDLLQQITEQRIKMARHANPLSGLPGNIPIQEELQRL
RQQGRAFYLAYFDLCHFKPYNDIYGFCRGDEVIQSLALRLQQQLGSEHFVGHVGGDDFVV
IGTSQAIVDECRRLLLSFNQDREAFYSPDDWLAQCVMAEDRQGQRVAHPLISLCVGLLPP
EVTRFCDEQGLSAYSAAAKKQAKAALNGFSLFTPAPNDAAIAMYRPLKFA