Protein Info for Shew_0416 in Shewanella loihica PV-4

Annotation: peptidase U62, modulator of DNA gyrase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 446 PF01523: PmbA_TldD_1st" amino acids 32 to 96 (65 residues), 47.9 bits, see alignment E=1.8e-16 PF19290: PmbA_TldD_2nd" amino acids 124 to 230 (107 residues), 66.8 bits, see alignment E=3.5e-22 PF19289: PmbA_TldD_3rd" amino acids 238 to 445 (208 residues), 226.4 bits, see alignment E=3.7e-71

Best Hits

Swiss-Prot: 60% identical to PMBA_ECO57: Metalloprotease PmbA (pmbA) from Escherichia coli O157:H7

KEGG orthology group: K03592, PmbA protein (inferred from 100% identity to slo:Shew_0416)

MetaCyc: 60% identical to metalloprotease subunit TldE (Escherichia coli)
3.4.24.-

Predicted SEED Role

"TldE protein, part of TldE/TldD proteolytic complex"

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3Q9Z0 at UniProt or InterPro

Protein Sequence (446 amino acids)

>Shew_0416 peptidase U62, modulator of DNA gyrase (RefSeq) (Shewanella loihica PV-4)
MASPSIETELNSLKDAVSMALEYAAKLGTSGAEVAISKQQGLSVSTRLKEVETVEFNKDG
ALGITLFRDGCKGSSSTSDLSPEAIRRAVKAADDIARFTSEDPFNGLADAELMAKQIEDL
ELYYPHETTPAELEALAARAETAALDADARIKNSDGASANAHTGAKVYGNSHGFLNGYVS
SRYSLSCVVIGEQDGHMQRDYDYTVARRFDDLWTPEAVGQQAAKKTLSRLGARKIATTKL
PILFAPDVATGLMGHLVGAISGSSLYRKSSFLLDAIDTQIFPEWFTIAEQPHLKGALASA
WYDSEGVATIDRNIIDSGVLSTYLLTSYSARKLGLTNTGHAGGIYNWTLSHTGQTFDELV
KQMGTGLIVTEVMGQGVNGVTGDYSRGAAGFYVENGEILYPVEEITIAGNLKEMYRNMVA
VGKEQDLRSSIRTGAILLDEMKIAGN