Protein Info for Shew_0340 in Shewanella loihica PV-4

Annotation: Ppx/GppA phosphatase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02541: Ppx-GppA
  • amino acids 23 to 305 (283 residues), 228.5 bits, E=5.9e-72

Best Hits

KEGG orthology group: K01524, exopolyphosphatase / guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase [EC: 3.6.1.11 3.6.1.40] (inferred from 100% identity to slo:Shew_0340)

Predicted SEED Role

"Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (EC 3.6.1.40)" in subsystem Phosphate metabolism or Stringent Response, (p)ppGpp metabolism (EC 3.6.1.40)

MetaCyc Pathways

• ppGpp metabolism (6/6 steps found)
• polyphosphate metabolism (2/2 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.11, 3.6.1.40

Use Curated BLAST to search for 3.6.1.11 or 3.6.1.40

Sequence Analysis Tools

See A3Q9R4 at UniProt or InterPro

Protein Sequence (310 amino acids)

>Shew_0340 Ppx/GppA phosphatase (RefSeq) (Shewanella loihica PV-4)
MKHSGVSPLYAAITLGSNSFNMLVAQTRAGKPTVVAKYKRKVRLAEGIGADGVLCPEVFD
RGLDCLQMFSQMLDHEGVSRDNIAVIATATLRSISNADAFCEAALPILGQPIEIISGLRE
AELIYQGMVATTVGEGRRLVIDIGGASTEFIIGDGNKVLLKTSLPMGCVTFKDKFFNSFP
FQQLDFDAAQSQVETALGDYRRRLTDYGWHCVVGASGTVQSVVELLNDRGVSATITPAVL
ADLRGEVLAQTDVSLRDIRGLHSERAPTFAAGLAILSALFNLLDIQSLNLSGGALREGVL
HQLAQRVLAA