Protein Info for Shew_0294 in Shewanella loihica PV-4

Annotation: alanine--glyoxylate transaminase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 42% identical to SPYA_CALJA: Serine--pyruvate aminotransferase, mitochondrial (AGXT) from Callithrix jacchus

KEGG orthology group: K00830, alanine-glyoxylate transaminase / serine-glyoxylate transaminase / serine-pyruvate transaminase [EC: 2.6.1.44 2.6.1.45 2.6.1.51] (inferred from 100% identity to slo:Shew_0294)

MetaCyc: 41% identical to Serine--pyruvate aminotransferase (Homo sapiens)
Serine--pyruvate transaminase. [EC: 2.6.1.51]; Alanine--glyoxylate transaminase. [EC: 2.6.1.51, 2.6.1.44]

Predicted SEED Role

"Serine--pyruvate aminotransferase (EC 2.6.1.51) / L-alanine:glyoxylate aminotransferase (EC 2.6.1.44)" in subsystem Photorespiration (oxidative C2 cycle) or Pyruvate Alanine Serine Interconversions or Serine-glyoxylate cycle (EC 2.6.1.44, EC 2.6.1.51)

MetaCyc Pathways

photorespiration II (8/10 steps found)
photorespiration I (7/9 steps found)
photorespiration III (7/9 steps found)
glycine biosynthesis III (1/1 steps found)
L-serine biosynthesis II (2/4 steps found)
formaldehyde assimilation I (serine pathway) (8/13 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.6.1.44 or 2.6.1.45 or 2.6.1.51

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3Q9L8 at UniProt or InterPro

Protein Sequence (380 amino acids)

>Shew_0294 alanine--glyoxylate transaminase (RefSeq) (Shewanella loihica PV-4)
MLAAPKVTPFNPPRRVLMGPGPSDVYPEVLAAQARPTIGHLDPLFVGMMDELKSLIQYAF
QTENAMTLAVSAPGSAGMETCFVNLVEPGEKVIVCRNGVFGERMRQNVERVGATAVVVDN
EWGTPVSLSDVEAALKAHPDAKFLAFVHAETSTGALSDAKGLCQLAKAHDCLSIVDAVTS
LGGVELRVDEWGIDAIYSGSQKCLSCVPGLSPVSFSPAAVEKLKARKTPVQSWFLDQSLV
MGYWSGDQGAGGKRSYHHTAPVNALYALHESLRLLAEEGLENAWARHHSMHALLRDGLEN
LGLKFVVEEGSRLPQLNAVYIPEGVDDGAVRKQLLENYNLEIGAGLGALAGKAWRIGLMG
FGARRENVALCLRALEEVLR