Protein Info for Shew_0020 in Shewanella loihica PV-4

Annotation: proline dipeptidase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 440 PF21216: PepQ_N" amino acids 7 to 155 (149 residues), 215.3 bits, see alignment E=4e-68 PF00557: Peptidase_M24" amino acids 166 to 425 (260 residues), 150 bits, see alignment E=8.1e-48

Best Hits

Swiss-Prot: 100% identical to PEPQ_SHELP: Xaa-Pro dipeptidase (pepQ) from Shewanella loihica (strain ATCC BAA-1088 / PV-4)

KEGG orthology group: K01271, Xaa-Pro dipeptidase [EC: 3.4.13.9] (inferred from 100% identity to slo:Shew_0020)

MetaCyc: 60% identical to Organophosphorus acid anhydrolase-2 (Alteromonas sp.)
3.8.2.a [EC: 3.8.2.a]

Predicted SEED Role

"Xaa-Pro dipeptidase PepQ (EC 3.4.13.9)" (EC 3.4.13.9)

Isozymes

Compare fitness of predicted isozymes for: 3.4.13.9

Use Curated BLAST to search for 3.4.13.9 or 3.8.2.a

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3Q8U5 at UniProt or InterPro

Protein Sequence (440 amino acids)

>Shew_0020 proline dipeptidase (RefSeq) (Shewanella loihica PV-4)
MENLATLYPAHIIELNRRVAEITAREQLAGLVIHSGQPHRQFLDDLDYPFKVNPHFKAWL
PVIDNPHCWLIVNGRDKPQLIFYRPVDFWHKVADLPEDFWTTEIEIKVLTKADKVADLLP
GKLQEWAYIGEHLDVADVLGFGSRNPEAVMSYLHYHRASKTAYELACMRRASEIGVRGHV
AAKSAFYAGASEFEIQQAYLAATDMGENDVPYGNIIALNQNAAILHYTALEHVSPKQRLS
FLIDAGGSFHGYASDITRTYAFEKNLFGDLIAAMDKLQLAIIEMMRPGVKYVDLHLATHQ
KLAQLLLDFKLVQGDPQGLIEQGITSAFFPHGLGHMLGLQVHDMGGFLHDERGTHIAPPE
AHPFLRCTRTLAANQVLTIEPGLYIIDSLLNELKQDGRADWINWQMVDQVRPFGGIRIED
NVIVHSDHNENMTRDLGLHG