Protein Info for DVU1918 in Desulfovibrio vulgaris Hildenborough JW710

Name: hysA
Annotation: periplasmic [NiFeSe] hydrogenase, large subunit, selenocysteine-containing (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF27537: HoxH
  • amino acids 18 to 47 (30 residues), 33.4 bits, see alignment (E = 3.9e-12)
• PF00374: NiFeSe_Hases
  • amino acids 53 to 132 (80 residues), 120.2 bits, E=1.3e-38
  • amino acids 135 to 495 (361 residues), 305.2 bits, E=1e-94

Best Hits

Swiss-Prot: 65% identical to PHSL_DESBA: Periplasmic [NiFeSe] hydrogenase large subunit from Desulfomicrobium baculatum

KEGG orthology group: K00532, ferredoxin hydrogenase [EC: 1.12.7.2] (inferred from 100% identity to dvu:DVU1918)

MetaCyc: 100% identical to cytochrome-c3 [NiFe]-hydrogenase large subunit (Desulfovibrio vulgaris)
Cytochrome-c3 hydrogenase. [EC: 1.12.2.1]

Predicted SEED Role

"[Ni/Fe] hydrogenase, group 1, large subunit; selenocysteine-containing"

MetaCyc Pathways

• lactate fermentation to acetate, CO₂ and hydrogen (Desulfovibrionales) (7/8 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (7/9 steps found)
• hydrogen production III (1/1 steps found)
• hydrogen production VIII (1/1 steps found)
• hydrogen production VI (1/2 steps found)
• superpathway of hydrogen production (1/2 steps found)
• superpathway of photosynthetic hydrogen production (2/6 steps found)
• L-glutamate degradation VII (to butanoate) (4/12 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Methane metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.12.7.2

Use Curated BLAST to search for 1.12.2.1 or 1.12.7.2

Sequence Analysis Tools

See Q72AS3 at UniProt or InterPro

Protein Sequence (510 amino acids)

>DVU1918 periplasmic [NiFeSe] hydrogenase, large subunit, selenocysteine-containing (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MSGCTPKAAPAGATGRTTIAIDPVTRIEGHLKAEVVVENGKVVDARLSGGMYRGFETILR
GRDPRDASQIVQRICGVCPTAHSTASVLALDEAFGAKVPNNGRITRNLIFGANYLQSHIL
HFYHLSAQDFVQGPDTAPFVPRFPKSDLRLSKELNKAGVDQYIEALEVRRICHEMVALFG
GRMPHVQGQVVGGATEIPTKEKLVEYAARFKKVRDFVEQKYVPVVYTIGSKYKDMFKVGQ
GFKAALCVGAFPLDNSGKKHLFMPGVYAKGKDMPFDPSKIKEYVKYSWFAEETTGLNYKE
GKTIPAPDKAGAYSFVKAPRYDGLSLEVGPLARMWVNNPELSPVGKKLLKDLFGISAKKF
RDLGEEAAFSLMGRHVARAEETYYMLGAIEGWLKEIKAGEDTVVMPAVPASAEGTGFTEA
PRGSLLHYVKVKDSKIDNYQIVSASLWNCNPRDDMGQRGAVEEALIGIPVDDIQNPVNVA
RLIRAFDPULGCAVHVLHAESGKVAVIEVK