Protein Info for DVU0748 in Desulfovibrio vulgaris Hildenborough JW710

Name: acs
Annotation: acetyl-CoA synthetase (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02188: acetate--CoA ligase
  • amino acids 33 to 646 (614 residues), 809.6 bits, E=8.1e-248
• PF16177: ACAS_N
  • amino acids 38 to 89 (52 residues), 63.5 bits, 1.9e-21
• PF00501: AMP-binding
  • amino acids 98 to 481 (384 residues), 296 bits, E=5.7e-92
• PF13193: AMP-binding_C
  • amino acids 541 to 622 (82 residues), 91.2 bits, E=9.7e-30

Best Hits

Swiss-Prot: 51% identical to ACSA_THET8: Acetyl-coenzyme A synthetase (acsA) from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)

KEGG orthology group: K01895, acetyl-CoA synthetase [EC: 6.2.1.1] (inferred from 100% identity to dvl:Dvul_2222)

MetaCyc: 49% identical to acetyl-CoA synthetase (Cupriavidus necator)
Propionate--CoA ligase. [EC: 6.2.1.17]; Acetate--CoA ligase. [EC: 6.2.1.17, 6.2.1.1]

Predicted SEED Role

"Acetyl-coenzyme A synthetase (EC 6.2.1.1)" in subsystem Ketoisovalerate oxidoreductase or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 6.2.1.1)

MetaCyc Pathways

• superpathway of acetate utilization and formation (3/3 steps found)
• acetate and ATP formation from acetyl-CoA III (1/1 steps found)
• acetate conversion to acetyl-CoA (1/1 steps found)
• L-isoleucine biosynthesis V (2/3 steps found)
• ethanol degradation II (2/3 steps found)
• ethanol degradation IV (2/3 steps found)
• L-isoleucine biosynthesis IV (4/6 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (6/9 steps found)
• chitin deacetylation (2/4 steps found)
• ethanol degradation III (1/3 steps found)
• superpathway of coenzyme A biosynthesis II (plants) (5/10 steps found)
• 2-methylcitrate cycle I (1/5 steps found)
• β-alanine biosynthesis II (1/6 steps found)
• 2-methylcitrate cycle II (1/6 steps found)
• adlupulone and adhumulone biosynthesis (1/6 steps found)
• colupulone and cohumulone biosynthesis (1/6 steps found)
• lupulone and humulone biosynthesis (1/6 steps found)
• cis-geranyl-CoA degradation (1/9 steps found)
• superpathway of bitter acids biosynthesis (3/18 steps found)

KEGG Metabolic Maps

• Glycolysis / Gluconeogenesis
• Propanoate metabolism
• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.1

Use Curated BLAST to search for 6.2.1.1 or 6.2.1.17

Sequence Analysis Tools

See Q72E31 at UniProt or InterPro

Protein Sequence (648 amino acids)

>DVU0748 acetyl-CoA synthetase (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MASPAATLDALLREERVFRPLPRVVADAVVNPQDVARAREQAGTAPLAFWEEAAEELEWF
RRWDGVLDDSNAPFMRWFPGALCNIVHNALDRHIETSNRHRLALIWEGEPGDSRRFTYFD
LYREVNRLANAMRSLGVTKGDRVVIYMPPLPETVFAMLACAKIGAVHSTVFGGFSARSLC
DRVEDARPTLLLTVDGFYRNGRVVPLKPVADDAVDALSPEARAGLHHVVVVHRAHLETPM
RDGRDIWYHDLVRGQSTEALTEVLSANDPLFVLYTSGTTGKPKGLVHSHGGYMVGVHRTM
RWVFDVRPTDIFWCTAEPGWITGHSYGVYGPLMAGTTTVLYEGHPLYPEAGRLWSMVERW
GVTILYTVPTVIRMLMRFGPLYPARHDITTLRLLATVGEPISPETWVWFYRTIGRGRCPV
LDTWWQTETGMIMVSPLPVSALKPGSVARPLPGVDVDIVDGEGAPVPPGRGGLLVVKNPW
PAMACGVFGDDEAFRRLYWERFPGWYCTGDVARRDDDGYIWIQGRADDVILIAGHRIGTA
EMEAALASHPAVAECAVIGVPDRLKGEVGKAFVVLRDTEEAAAEEQALATSLVGHVRREL
GPVAVIRDVVFREGLPRNRSGKIMRRILRSEEMGVETGDLSTLEDAYI