Protein Info for Dshi_4221 in Dinoroseobacter shibae DFL-12

Annotation: 2-hydroxy-3-oxopropionate reductase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 293 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details PF03446: NAD_binding_2" amino acids 3 to 157 (155 residues), 126.2 bits, see alignment E=3.2e-40 PF02737: 3HCDH_N" amino acids 4 to 41 (38 residues), 22.4 bits, see alignment 2.5e-08 PF03807: F420_oxidored" amino acids 4 to 60 (57 residues), 22.1 bits, see alignment E=5e-08 PF14833: NAD_binding_11" amino acids 161 to 281 (121 residues), 116.7 bits, see alignment E=2e-37

Best Hits

Swiss-Prot: 53% identical to LTND_CUPNH: L-threonate dehydrogenase (ltnD) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K00042, 2-hydroxy-3-oxopropionate reductase [EC: 1.1.1.60] (inferred from 100% identity to dsh:Dshi_4221)

MetaCyc: 43% identical to L-threonate 2-dehydrogenase (Haemophilus influenzae Rd KW20)
RXN-18590 [EC: 1.1.1.411]

Predicted SEED Role

"2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60)" in subsystem Allantoin Utilization or D-galactarate, D-glucarate and D-glycerate catabolism or Photorespiration (oxidative C2 cycle) (EC 1.1.1.60)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.60

Use Curated BLAST to search for 1.1.1.411 or 1.1.1.60

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LUL4 at UniProt or InterPro

Protein Sequence (293 amino acids)

>Dshi_4221 2-hydroxy-3-oxopropionate reductase (RefSeq) (Dinoroseobacter shibae DFL-12)
MSDVAIIGLGSMGYGMAQSLLRAGLRVYGADIAPEPMARFRAEGGLAARPEAVDILVIAV
LNAAQTEAVLFGAEGWISTLRKGGVVIACATVPPRFARDMAARCAEAGVQYLDAPISGGA
AKAAQGRLSIMASGGAEAFAAAAPALDAMAETVFELGDAPGAGSAMKAINQMLAGTHIAA
MAEAVTFGMTQGVDPAKTVEVISRCAGTSWMFENRAPHIVEGDYTPRSSVNIWPKDLGIV
LDIAAEAKFAAPLTAAALQQFMAAAGSGLGGEDDAAVAKVYARNAGLTLPGEG