Protein Info for Dshi_4200 in Dinoroseobacter shibae DFL-12

Annotation: Carbon-monoxide dehydrogenase (acceptor) (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 277 PF00941: FAD_binding_5" amino acids 7 to 174 (168 residues), 160.3 bits, see alignment E=3.9e-51 PF03450: CO_deh_flav_C" amino acids 184 to 271 (88 residues), 47.4 bits, see alignment E=1.9e-16

Best Hits

KEGG orthology group: K03519, carbon-monoxide dehydrogenase medium subunit [EC: 1.2.99.2] (inferred from 100% identity to dsh:Dshi_4200)

Predicted SEED Role

"Carbon monoxide dehydrogenase medium chain (EC 1.2.99.2)" in subsystem CO Dehydrogenase (EC 1.2.99.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.99.2

Use Curated BLAST to search for 1.2.99.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LUJ3 at UniProt or InterPro

Protein Sequence (277 amino acids)

>Dshi_4200 Carbon-monoxide dehydrogenase (acceptor) (RefSeq) (Dinoroseobacter shibae DFL-12)
MKASAGGYARANDLREALDLLANADGQGRVLAGGQSLIAAMNMRLSTGDMLVDISRIAEL
AGVAEDGETLRIGALTRHAAVGADPLVKTHVPLLAEATGHIAHAAIRNRGTIGGALSHAD
PAAEFPACALALGASMEIAGPDGSRTVAAEDFFEDVFTTALAEGEILTALTVPKQRPGEA
QLIEEVARRSGDYALVGLCLVKRDAGHRVALFSVGATPILARACMARLDAGDLDGAVTAL
QGEIDPPSDTQASAAYRRHLAGVLLRRAMARLTGVPA