Protein Info for Dshi_4114 in Dinoroseobacter shibae DFL-12

Annotation: dTDP-4-dehydrorhamnose 3,5-epimerase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01221: dTDP-4-dehydrorhamnose 3,5-epimerase
  • amino acids 3 to 175 (173 residues), 198.1 bits, E=5.1e-63
• PF00908: dTDP_sugar_isom
  • amino acids 6 to 175 (170 residues), 216.7 bits, E=8.4e-69

Best Hits

Swiss-Prot: 51% identical to RMLC_SALTY: dTDP-4-dehydrorhamnose 3,5-epimerase (rfbC) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K01790, dTDP-4-dehydrorhamnose 3,5-epimerase [EC: 5.1.3.13] (inferred from 100% identity to dsh:Dshi_4114)

MetaCyc: 45% identical to dTDP-4-dehydrorhamnose 3,5-epimerase (Escherichia coli K-12 substr. MG1655)
dTDP-4-dehydrorhamnose 3,5-epimerase. [EC: 5.1.3.13]

Predicted SEED Role

"dTDP-4-dehydrorhamnose 3,5-epimerase (EC 5.1.3.13)" in subsystem Capsular heptose biosynthesis or Rhamnose containing glycans or dTDP-rhamnose synthesis or linker unit-arabinogalactan synthesis (EC 5.1.3.13)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• dTDP-β-L-rhamnose biosynthesis (5/5 steps found)
• dTDP-4-O-demethyl-β-L-noviose biosynthesis (3/5 steps found)
• dTDP-L-daunosamine biosynthesis (3/6 steps found)
• dTDP-sibirosamine biosynthesis (3/6 steps found)
• superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis (5/19 steps found)
• superpathway of novobiocin biosynthesis (4/19 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (13/33 steps found)

KEGG Metabolic Maps

• Polyketide sugar unit biosynthesis
• Streptomycin biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.3.13

Sequence Analysis Tools

See A8LUB4 at UniProt or InterPro

Protein Sequence (184 amino acids)

>Dshi_4114 dTDP-4-dehydrorhamnose 3,5-epimerase (RefSeq) (Dinoroseobacter shibae DFL-12)
MQIEDTPLPGVKLLTPARFGDARGFFSESYSRRVLAGHGITLDFVQDNHSLSAQVGTVRG
LHFQAPPHAQDKLVRCGRGRLFDVAVDVRRGSPTYGQWFGAELSFENGKQLLVPAGFLHG
FVTREPDTEIVYKCSDYYAPECDGAVHWDSCGIDWGFEGDPVISAKDAAAVRLADFDSPF
VYEG