Protein Info for Dshi_3681 in Dinoroseobacter shibae DFL-12
Annotation: thiamine biosynthesis protein ThiC (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 86% identical to THIC_RHIME: Phosphomethylpyrimidine synthase (thiC) from Rhizobium meliloti (strain 1021)
KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 100% identity to dsh:Dshi_4080)MetaCyc: 77% identical to HMP-P synthase monomer (Caulobacter vibrioides)
PYRIMSYN1-RXN [EC: 4.1.99.17]
Predicted SEED Role
No annotation
MetaCyc Pathways
- 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
- superpathway of thiamine diphosphate biosynthesis II (8/11 steps found)
- superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (4/7 steps found)
- superpathway of thiamine diphosphate biosynthesis I (6/10 steps found)
Isozymes
Compare fitness of predicted isozymes for: 4.1.99.17
Use Curated BLAST to search for 4.1.99.17
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A8LT47 at UniProt or InterPro
Protein Sequence (602 amino acids)
>Dshi_3681 thiamine biosynthesis protein ThiC (RefSeq) (Dinoroseobacter shibae DFL-12) MKDLTPTVTTGPLPASRRVYQPGTLHPQIRVPMREIDLHPSAGEPPVTVYDSSGPYTDPS AKIAINGGLPRLRADWIAARNDIELYEGRHVRPEDNGFAEGARLVPEFPVRHKPLQAKAG RAVTQMAYARAGIITPEMEFVAIRENLGRKAAKEALVRDGQDWGAAIPDFVTPDFVREEV ARGRAIIPANINHPEAEPMGIGRNFLVKINANIGNSAVTSSMEEEVEKMVWATRWGADTV MDLSTGRNIHNIRDWIIRNSAVPIGTVPLYQALEKVEGIAEDLSWEVYRDTLIEQAEQGV DYFTIHAGVRLHMIPMTIKRVTGIVSRGGSIMAKWCLHHHRESFLYEHFAEICEIARAYD VSFSLGDGLRPGSIADANDEAQFAELETLGELTQIAWKHDCQVMIEGPGHVAMHKIKENM DKQLEVCGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPNR DDVKIGVITYKIAAHAADLAKGHPGAQIRDDALSRARFEFRWEDQFNLSLDPETARSMHD ETLPKEAHKVAHFCSMCGPKFCSMRISHDIRAEAQKDGMAKMAEKFRKGGDLYVPLDKVK EI