Protein Info for Dshi_3655 in Dinoroseobacter shibae DFL-12

Annotation: TRAG family protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 667 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details transmembrane" amino acids 62 to 83 (22 residues), see Phobius details amino acids 346 to 367 (22 residues), see Phobius details PF02534: T4SS-DNA_transf" amino acids 127 to 541 (415 residues), 288.7 bits, see alignment E=1.3e-89 PF10412: TrwB_AAD_bind" amino acids 344 to 521 (178 residues), 53.1 bits, see alignment E=3.9e-18 PF12696: TraG-D_C" amino acids 386 to 507 (122 residues), 100.7 bits, see alignment E=9.2e-33

Best Hits

KEGG orthology group: K03205, type IV secretion system protein VirD4 (inferred from 100% identity to dsh:Dshi_3655)

Predicted SEED Role

"Coupling protein VirD4, ATPase required for T-DNA transfer"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LT21 at UniProt or InterPro

Protein Sequence (667 amino acids)

>Dshi_3655 TRAG family protein (RefSeq) (Dinoroseobacter shibae DFL-12)
MGKARIATGVFLVTLVTGAMGYTIASAVLTYQDLGFGAEIDFAYIAQNYMAILDRRPEDA
QLIHLIIGSFAAAGLMLSLALSGSALTRFGQTHWQTAREMKANGFFGAPGTGFILGKLGP
PGSRANYICSKVFPHALIVAPTGRGKTTGFVIPNLLTWQGSAVTLDVKGECFEATARHRA
AQGDKVYRFAPTDWEGKRTHRYNPLLRIYQLKDPARQQMELQLLATLFLQSDNDRVQGLL
KGGIDLFVAAGLLAFQRKRPTLGEIYRIAASGGNKQKEYFARGHEVDNRAAKLIFTRLAS
TNNDTLTSYVSLLMTSGLDQWQNPAIDEATAVSDFDFRTIRKKPFSVYLVVQPLMVKPLA
PLIRLFFSDLLSAMQEKDPGPDEPWPVMIMLDEFNRLGKMPIVVESIETLRTYRGHLAVV
TQTIPALDEIYGENTRRALQGNAGVKLYLTPSDEKTVEELSKAVGKTTKTVVTRSQSIGK
NPFEGRSQSTRTEESSLLPEDEARRLPLDEIVMVIDAQMPVRAKRIQYFDDRLFKAIHAA
QTGELPFPEPGGGGPQGTLPLSMRAMPMTPPANGPGGSDAVVETTRQEAGSQPSGQTDVA
LKKTAPVVLAVIAEEQRQMEMDFGGQVADAETVGVDDEAQLRSAVDGLDDMEAMLQEGDG
GEKLVAR