Protein Info for Dshi_3467 in Dinoroseobacter shibae DFL-12

Annotation: Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+))., Phosphate acetyltransferase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00390: malic
  • amino acids 22 to 154 (133 residues), 97.7 bits, E=1.1e-31
• PF03949: Malic_M
  • amino acids 168 to 364 (197 residues), 80.1 bits, E=2.6e-26
• PF01515: PTA_PTB
  • amino acids 432 to 748 (317 residues), 248.6 bits, E=1.6e-77

Best Hits

Swiss-Prot: 60% identical to MAO2_RHIME: NADP-dependent malic enzyme (tme) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00029, malate dehydrogenase (oxaloacetate-decarboxylating)(NADP+) [EC: 1.1.1.40] (inferred from 100% identity to dsh:Dshi_3467)

Predicted SEED Role

"NADP-dependent malic enzyme (EC 1.1.1.40)" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP (EC 1.1.1.40)

MetaCyc Pathways

• gluconeogenesis I (11/13 steps found)
• C4 photosynthetic carbon assimilation cycle, NADP-ME type (4/7 steps found)
• methylgallate degradation (2/6 steps found)
• protocatechuate degradation I (meta-cleavage pathway) (3/8 steps found)
• C4 photosynthetic carbon assimilation cycle, PEPCK type (7/14 steps found)
• superpathway of vanillin and vanillate degradation (4/10 steps found)
• syringate degradation (3/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of terpenoids and steroids
• Carbon fixation in photosynthetic organisms
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.40

Use Curated BLAST to search for 1.1.1.40

Sequence Analysis Tools

See A8LPD5 at UniProt or InterPro

Protein Sequence (752 amino acids)

>Dshi_3467 Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+))., Phosphate acetyltransferase (RefSeq) (Dinoroseobacter shibae DFL-12)
MSGNNKITREEALAYHLEPTPGKLEVSASTPMSTQRDLSLAYSPGVAVPCEAIAENPETA
YDYTTKGNLVAVITNGSAVLGLGNLGALASKPVMEGKSVLFKRFADVNSIDIELDTEDPD
AIIQAVKLMAPTFGGVNLEDIKAPECFIIEQTLKEQLDIPVFHDDQHGTAVITAAGLLNA
LRLTGKAIEDCKIVLNGAGAAGIACIELIKAMGAKPQNCIVCDTKGVIFQGRTEGMNQWK
SAHAVKTEMRTLDDAMKGADVFLGVSVKGAVTQAMLESMADDPVIFAMANPDPEITPEEA
HAIRPDAIVATGRSDYPNQVNNVLGFPYLFRGALDIRARAINDEMKIACAEALADLARED
VPDEVALAYGRKLSFGRDYIIPTPFDPRLIHTVPLAVAKAGMDTGVARRPIVDMDGYEQS
LRARMDPTASMLQGMFARAKGTQARMIFAEGDDIRVLRAAVTYQRSGLGKALVVGRQDDV
KTRLEAAGIGDAFRELEIVNAGNTRHLETYKEFLYGRLQRKGYDTSDIHRLAARDRHAFS
ALMLAHGHGDGLVTGATRKSAHVLGLINTVFDAGAEDGAVGVTALLVKGRIVLIADTLVH
EWPDEEDLADIATRAAGTARSLGLEPRVAFVSFSTFGYPVSERATKMHRAPRVLDARGVD
FEYDGEMTVDVALDPEIMAQYPFCRLSGPANVLVVPARHSASISVKLMQQLGDATVIGPI
LSGVGKPIQLCSATSTANDILNMAVLAACRVG