Protein Info for Dshi_3426 in Dinoroseobacter shibae DFL-12

Annotation: adenosylhomocysteinase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 88% identical to SAHH_ROSDO: Adenosylhomocysteinase (ahcY) from Roseobacter denitrificans (strain ATCC 33942 / OCh 114)

KEGG orthology group: K01251, adenosylhomocysteinase [EC: 3.3.1.1] (inferred from 100% identity to dsh:Dshi_3426)

Predicted SEED Role

"Adenosylhomocysteinase (EC 3.3.1.1)" in subsystem Methionine Biosynthesis or Methionine Degradation (EC 3.3.1.1)

MetaCyc Pathways

S-adenosyl-L-methionine salvage II (3/3 steps found)
L-methionine degradation I (to L-homocysteine) (2/3 steps found)
superpathway of L-cysteine biosynthesis (mammalian) (3/5 steps found)
superpathway of L-methionine salvage and degradation (10/16 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.3.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LP94 at UniProt or InterPro

Protein Sequence (462 amino acids)

>Dshi_3426 adenosylhomocysteinase (RefSeq) (Dinoroseobacter shibae DFL-12)
MAKDYIVKDIALADYGRKELDIAETEMPGLMALREQYGESKPLSGARIVGSLHMTIQTAV
LIETLTALGADVRWASCNIYSTQDHAAAAIAAGGTPVFAIKGQTLEEHWDYLDKSFMFPD
GPNMILDDGGDATLYVLLGARAEAGEDIIPVPSSEEEEVIKAQIAKRMSASPGWFTKTRD
QIKGVSEETTTGVHRLYELMRDGQLPFPAINVNDSVTKSKFDNKYGCKESLVDGIRRATD
TMMAGKVAVVCGYGDVGKGSAASLRGAGARVKVTEIDPICALQAAMDGFEVVTLEDTVKD
ADIFITTTGNKDVIRIEHMREMKDMAIVGNIGHFDNEIQVAALKNHKWTNIKDQVDMIEM
PSGNRMILLSQGRLLNLGNATGHPSFVMSASFTNQVLAQIELWTKGDQYGNEVYVLPKHL
DEMVAKLHLAKVGAKLTELAKEQADYIGVSQDGPYKPEHYRY